| Project/Area Number |
26670389
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
渡邊 嘉行 聖マリアンナ医科大学, 医学部, 講師 (90329243)
伊東 文生 聖マリアンナ医科大学, 医学部, 教授 (90223180)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | HPV / 食道癌 / ゲノム / エピゲノム / オミクス解析 |
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| Outline of Final Research Achievements |
Integration of DNA viruses into the human genome plays an important role in various types of tumors. However, the molecular details and clinical impact of HPV integration on either the human or human papilloma virus (HPV) epigenomes in esophageal squamous cell carcinoma (ESCC) are unknown. We used a next-generation sequencing-based method for structural methylation analysis of integrated viral genomes. We detected integrated HPV sequences in the genome of the ESCC cell line and found variable levels of methylation within the integrated HPV genomes. The observed dynamic changes in DNA methylation of the host and viral genomes may functionally affect the biological behavior of HPV.
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