Regulation of differentiation, regeneration, and homeostasis via signaling-epigenome interaction

• Project/Area Number: 26670401
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kyoto University
• Principal Investigator: JUN YAMASHITA 京都大学, iPS細胞研究所, 教授 (50335288)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 多能性幹細胞 / 分化 / エピジェネティクス / 液性因子 / 発生分化 / エピゲノム / シグナル

Outline of Final Research Achievements

Purpose of this study is development of novel manipulating methods of epigenome through identification of signaling molecules being involved in epigenetic regulation.
We identified a humoral factor that regulate differentiation velocity in early differentiation stage of pluripotent stem cells through epigenetic silencing of pluripotent genes.Now we are investigating other physiological functions of the gene than differentiation through epigenetic regulation.

Research Products

• [Journal Article] Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart (2014)
  • Author(s): 3.Arita Y, Nakaoka Y, Matsunaga T, Kidoya H, Yamamizu K, Arima Y, Kataoka-Hashimoto T, Ikeoka K, Yasui T, Masaki T, Yamamoto K, Higuchi K, Park JS, Shirai M, Nishiyama K, Yamagishi H, Otsu K, Kurihara H, Minami T, Yamauchi-Takihara K, Koh GY, Mochizuki N, Takakura N, Sakata Y, Yamashita JK, Komuro I.
  • Journal Title: Nat Commun Volume: 5 Pages: 4552-4552
  • Peer Reviewed / Open Access
• [Journal Article] Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration. (2014)
  • Author(s): Masumoto H, Ikuno T, Takeda M, Fukushima H, Marui A, Katayama S, Shimizu T, Ikeda T, Okano T, Sakata R, Yamashita JK.
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 6716-6716
  • DOI: 10.1038/srep06716
  • Peer Reviewed / Open Access
• [Presentation] 2型VEGF受容体陽性細胞純化再培養はヒトiPS細胞からの血管内皮細胞分化誘導を効率化する. (2015)
  • Author(s): 幾野 毅
  • Organizer: 第18回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2015-03-19
• [Presentation] 2型VEGF受容体陽性細胞純化再培養によるヒトiPS細胞からの効率的血管内皮細胞分化誘導. (2015)
  • Author(s): 幾野 毅
  • Organizer: 第45回日本心臓血管外科学会学術総会.
  • Place of Presentation: 京都
  • Year and Date: 2015-02-18
• [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells based on monolayer and serum-free culture for realization of regenerative medicine. (2015)
  • Author(s): 幾野 毅
  • Organizer: The 18th Takeda Science Foundation Symposium on Bioscience,
  • Place of Presentation: 大阪
  • Year and Date: 2015-01-15 – 2015-01-17
• [Presentation] ２型血管内皮細胞増殖因子受容体陽性細胞の純化再培養によるヒトiPS 細胞からの血管内皮細胞分化誘導の効率化 (2014)
  • Author(s): 幾野 毅
  • Organizer: 第67回日本胸部外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-10-03
• [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells for realization of regenerative medicine． (2014)
  • Author(s): 幾野 毅
  • Organizer: 第5回Molecular Cardiovascular ConferenceⅡ.
  • Place of Presentation: Kobe
  • Year and Date: 2014-09-06
• [Presentation] ヒトiPS細胞由来心筋特異的前駆細胞の同定 (2014)
  • Author(s): 武田 匡史、山下 潤
  • Organizer: 第5回 Molecular Cardiovascular Conference Ⅱ
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells based on monolayer and serum-free culture. (2014)
  • Author(s): Ikuno T.
  • Organizer: ESC Congress2014.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2014-08-31
• [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells based on monolayer and serum-free culture for realization of regenerative medicine. (2014)
  • Author(s): Ikuno T.
  • Organizer: International Society for Stem Cell Research (ISSCR) 12th Annual Meeting.
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2014-06-20
• [Presentation] Identification of novel chemicals potently inducing cardiomyocyte differentiation from mouse and human pluripotent stem cells (2014)
  • Author(s): Fukushima H.
  • Organizer: ISSCR (International Society for Stem Cell Research) 12th Annual Meeting
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2014-06-18 – 2014-06-21
• [Presentation] Identification and application of human iPS derived-cardiac progenitor enriched population for cardiac regeneration. (2014)
  • Author(s): Takeda T, Yamashita JK.
  • Organizer: The 3ird Retreat of Center for iPS Cell Research and Application (CiRA)
  • Place of Presentation: Shiga
  • Year and Date: 2014-04-19
• [Remarks] 山下研究室のホームページ
  • URL: http://www.cira.kyoto-u.ac.jp/yamashita/achievement.html