| Project/Area Number |
26670416
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
Sato Mitsuo 名古屋大学, 医学部附属病院, 講師 (70467281)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Masashi 名古屋大学, 大学院医学系研究科, 准教授 (00378077)
HASE Tetsunari 名古屋大学, 医学部附属病院, 病院助助 (30621635)
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| Co-Investigator(Renkei-kenkyūsha) |
YUKAWA Hiroshi 名屋大学, 大学院工学系研究科, 特任講師 (30634646)
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| Research Collaborator |
YOGO Naoyuki 名古屋大学, 大学院医学系研究科, 博士過程3年生
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | EGFR遺伝子変異 / RNA干渉 / プールshRNA / EGFR 遺伝子変異 / クラスター形成 / アポトーシス |
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| Outline of Final Research Achievements |
This study is aimed to test the hypothesis that EGFR mutated lung cancer cells complete a metastatic process by maintaining epithelial characteristics through expressing adhesion molecules. To identify genes contributing to forming clustering, we performed a screen with pooled shRNA library. The library was lentivirally transduced in immortalized normal bronchial epithelial cell lines (HBEC) and then the HBEC were grown in 2D or 3D culture condition. Comparing amount of shRNA between the two conditions by next generation sequencing, we identified potential genes contributing to forming cluster. To confirm this result, we also did individual knockdown of the identified genes. Through these experiments, we were able to identify genes that could serve as therapeutic target in EGFR mutated lung cancer.
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