| Project/Area Number |
26670422
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Keio University |
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Principal Investigator |
SAITO Fumitake 慶應義塾大学, 医学部(信濃町), 共同研究員 (30338040)
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| Research Collaborator |
YOTSUKURA Masaya 慶應義塾大学, 医学部・呼吸器外科, 助教
ISHII Makoto 慶應義塾大学, 医学部, 講師
IEDA Masaki 慶應義塾大学, 医学部, 講師
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 再生医療 / リプログラミング / 肺胞上皮 / 直接リプログラミング / 肺上皮細胞 / 転写因子 / 再生医学 / 分化誘導 / ES細胞 / 線維芽細胞 |
|---|
| Outline of Final Research Achievements |
Direct reprogramming has been reported recently as one of the new methods of regenerative medicine. By direct reprogramming method, somatic cells are converted into other types of cells directly, not using stem cells. We induced 3 or 4 selected factors into fibroblasts of mice. After culturing, the induced cells were found to express Surfactant Protein C, which is a specific marker of type II alveolar cell (AT II) by immunofluorescence staining. The presence of “lamellar body” , which is known as a specific structure of AT II, was detected in the cytoplasm of these induced cells by electron microscopy. These results suggested that induced AT II was obtained by direct reprogramming method.
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