| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Previous methods to evaluate oxygenation of organs measured oxygen tensions in the blood stream of organs, and no method could evaluate intracellular oxygenation. We employed the lipophilic phosphofluorescence probe, which is quickly taken up by tubular cells in the kidney. Utilizing this probe, we established the method to measure intracellular oxygen tensions accurately by calculating half-life of the probe. This method allowed us to prove changes of intracellular oxygen tensions of tubular cells in a variety of disease models. Hypoxia is the final common pathway of chronic kidney disease, and this method will serve as a powerful tool to develop novel drugs against kidney disease.
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