Development of the methodology to evaluate oxygenation of organs in vivo utilizing organic luminescence

• Project/Area Number: 26670425
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: Nangaku Masaomi 東京大学, 医学部附属病院, 教授 (90311620)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 低酸素 / 腎臓 / りん光プローブ / 腎臓学 / 慢性腎臓病 / 酸素分圧 / 有機ルミネッセンス

Outline of Final Research Achievements

Previous methods to evaluate oxygenation of organs measured oxygen tensions in the blood stream of organs, and no method could evaluate intracellular oxygenation. We employed the lipophilic phosphofluorescence probe, which is quickly taken up by tubular cells in the kidney. Utilizing this probe, we established the method to measure intracellular oxygen tensions accurately by calculating half-life of the probe. This method allowed us to prove changes of intracellular oxygen tensions of tubular cells in a variety of disease models. Hypoxia is the final common pathway of chronic kidney disease, and this method will serve as a powerful tool to develop novel drugs against kidney disease.

Research Products

• [Int'l Joint Research] Ｕｐｐｓａｌａ Ｕｎｉｖｅｒｓｉｔｙ(Ｓｗｅｄｅｎ)
• [Journal Article] Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement (2015)
  • Author(s): Hirakawa Y, Yoshihara T, Kamiya M, Mimura I, Fujikura D, Masuda T, Kikuchi R, Takahashi I, Urano Y, Tobita S, Nangaku M.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 17838-17838
  • DOI: 10.1038/srep17838
  • Peer Reviewed / Open Access
• [Presentation] Quantitating Intracellular Oxygen Tension in Kidney by Phosphorescence Lifetime Measurement. (2015)
  • Author(s): Yosuke Hirakawa, Imari Mimura, Toshitada Yoshihara, Mako Kamiya, Yasuteru Urano, Seiji Tobita, Masaomi Nangaku.
  • Organizer: The 49th Annual Meeting of the American Society of Nephrology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-11-07
  • Int'l Joint Research
• [Presentation] Oxidative Stres and Kidney Injury. (2015)
  • Author(s): Nangaku M.
  • Organizer: The 49th Annual Meeting of the American Society of Nephrology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-11-06
  • Int'l Joint Research / Invited
• [Presentation] Estimation of intracellular oxygen concentration in diabetic kidney by phosphorescence lifetime measurement. (2015)
  • Author(s): Hirakawa Y, Mimura I, Yoshihara T, Kamiya M, Tanaka S, Tanaka T, Inagi R, Urano Y, Tobita S, Nangaku M.
  • Organizer: Keystone Symposia: Diabetes: New insights into molecular mechanisms and therapeutic strategies.
  • Place of Presentation: ウエスティン都ホテル京都（京都府京都市）
  • Year and Date: 2015-10-26
  • Int'l Joint Research