Functional analysis of glomerular podocytes differentiated from iPS cells established from patients with kidney disease
Project/Area Number |
26670429
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Kidney internal medicine
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Research Institution | Niigata University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
後藤 眞 新潟大学, 医歯学系, 准教授 (00463969)
金子 佳賢 新潟大学, 医歯学系, 講師 (80444157)
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Research Collaborator |
SATO Yuya
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | ネフローゼ症候群 / iPS細胞 / 糸球体上皮細胞 / プロラクチン / 性差 / 遺伝性ネフローゼ症候群 / Tリンパ球 / T細胞 / 初期化遺伝子 / FSGS / MCNS |
Outline of Final Research Achievements |
The purpose of this study was to establish iPS cells from peripheral T-cells of healthy volunteers or patients with hereditary nephrotic syndrome, and to introduce differentiation into glomerular podocyte,and investigate their functions. We succeeded to establish the iPS cells and found that the differentiated cells from these iPSs were positive with nephrin, podocin, synaptopodin, podocalyxin. However, any functional difference was not observed on cells originated from patients with hereditary nephrotic syndrome as compared to those from healthy controls, in respect with the response to renin-angiotensin, inflammatory cytokines, etc. In parallel, we also demonstrated that male-predominant Mups expression and MUPs production and urine excretion were down-regulated, whereas female-predominant genes such as Cyp3a41 and Cyp3a44 expressions were up-regulated by prolactin, and that the regulatory role of prolactin was independent with sex-specific GH secretion pattern or testosterone.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Megalin Blockade with Cilastatin Suppresses Drug-Induced Nephrotoxicity2017
Author(s)
Hori Y, Aoki N, Kuwahara S, Hosojima M, Kaseda R, Goto S, Iida T, De S, Kabasawa H, Kaneko R, Aoki H, Tanabe Y, Kagamu H, Narita I, Kikuchi T, Saito A.
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Journal Title
J Am Soc Nephrol
Volume: in press
Issue: 6
Pages: 1783-1791
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Exocytosis-Mediated Urinary Full-Length Megalin Excretion is Linked with the Pathogenesis of Diabetic Nephropathy2017
Author(s)
De S, Kuwahara S, Hosojima M, Ishikawa T, Kaseda R, Sarkar P, Yoshioka Y, Kabasawa H, Iida T, Goto S, Toba K, Higuchi Y, Suzuki Y, Hara M, Kurosawa H, Narita I, Hirayama Y, Ochiya T, Saito A
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Journal Title
Diabetes
Volume: in press
Issue: 5
Pages: 1391-1404
DOI
Related Report
Peer Reviewed
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[Journal Article] Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy2017
Author(s)
Watanabe H, Goto S, Mori H, Higashi K, Hosomichi K, Aizawa N, Takahashi N, Tsuchida M, Suzuki Y, Yamada T, Horii A, Inoue I, Kurokawa K, Narita I.
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Journal Title
Nephrol Dial Transplant
Volume: in press
Pages: 2072-2079
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Oral pharmacological chaperone migalastat compared with enzyme replacement therapy in Fabry disease: 18-month results from the randomised phase III ATTRACT study2017
Author(s)
Hughes DA, Nicholls K, Shankar SP, Ohashi T, Olivotto I, Sakai N, Eyskens F, Germain DP, Goker-Alpan O, Hachulla E, Jovanovic A, Lourenco CM, Narita I, Thomas M,
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Journal Title
J Med Genet
Volume: 54
Issue: 4
Pages: 288-296
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Megalin-Mediated Tubuloglomerular Alterations in High-Fat Diet-Induced Kidney Disease2016
Author(s)
Kuwahara S, Hosojima M, Kaneko R, Aoki H, Nakano D, Sasagawa T, Kabasawa H, Kaseda R, Yasukawa R, Ishikawa T, Suzuki A, Sato H, Kageyama S, Tanaka T, Kitamura N, Narita I, Komatsu M, Nishiyama A, Saito A
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Journal Title
J Am Soc Nephro
Volume: in press
Issue: 7
Pages: 1996-2008
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Leptin deficiency down-regulates IL-23 production in glomerular podocytes resulting in an attenuated immune response in nephrotoxic serum nephritis.2015
Author(s)
Goto K, Kaneko Y, Sato Y, Otsuka T, Yamamoto S, Goto S, Yamamoto K, Yamamoto T, Kawachi H, Madaio MP, Narita I
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Journal Title
Int Immunol
Volume: 28
Issue: 4
Pages: 197-208
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Two Forms of Urinary Megalin Excretion Are Novel Predictors of the Progression of Early-Stage Diabetic Nephropathy in Type 2 Diabetes Mellitus.2016
Author(s)
Iida T, Hosojima M, Kabasawa K, Nakamura K, Kuwahara S, Ishikawa T, Kaseda R, Suzuki Y, Narita I, Saito A.
Organizer
American Society of Nephrology 2016
Place of Presentation
Convention Center, Chicago, IL, USA
Year and Date
2016-11-17
Related Report
Int'l Joint Research
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[Presentation] Protein-Bound Uremic Toxin Adsorption by Hexadecyl-Immobilized Cellulose Beads in Hemodialysis.2016
Author(s)
Yamamoto S, Sato M, Wakamatsu T, Takahashi Y, Iguchi A, Omori K, Suzuki Y, Ei I, Kazama J, Gejyo F, Narita I
Organizer
American Society of Nephrology 2016,
Place of Presentation
Convention Center, Chicago, IL, USA
Year and Date
2016-11-17
Related Report
Int'l Joint Research
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[Presentation] Renin-Angiotensin System Inhibition Ameliorates Bone Fragility Through Blocking Both Osteocytic and Osteoblastic Actions in Uremic Rats.2016
Author(s)
Wakamatsu T, Iwasaki Y, Yamamoto S, Ito A, Takahashi Y, Gejyo F, Kazaa J, Fukagawa M, Narita I
Organizer
American Society of Nephrology 2016
Place of Presentation
Convention Center, Chicago, IL, USA
Year and Date
2016-11-17
Related Report
Int'l Joint Research
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[Presentation] Mechanism of Increased Urinary Full-Length Megalin Excretion in Type 2 Diabetes Mellitus Patients with Nephropathy2015
Author(s)
Shankhajit De, Shoji Kuwahara, Michihiro Hosojima, Tomomi Ishikawa, Ryohei Kaseda, Yusuke Yoshioka, Yoshiki Suzuki, Ichiei Narita, Takahiro Ochiya, Akihiko Saito
Organizer
American Society of Nephrology Kidney Week 2015
Place of Presentation
Convention Center, San Diego, USA
Year and Date
2015-11-03
Related Report
Int'l Joint Research
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[Book] Is IgAN a familial or sporadic disease? in Pathogenesis and Treatment in IgA Nephropathy, Tomino Y, ed.2016
Author(s)
Narita I, Kaneko Y, Itho Y, Sakamaki Y, Iguchi S, Yamamoto S, Wakasugi M, Kazama JJ, Goto S.
Publisher
Springer
Related Report
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