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Functional analysis of glomerular podocytes differentiated from iPS cells established from patients with kidney disease

Research Project

Project/Area Number 26670429
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

NARITA Ichiei  新潟大学, 医歯学系, 教授 (20272817)

Co-Investigator(Kenkyū-buntansha) 後藤 眞  新潟大学, 医歯学系, 准教授 (00463969)
金子 佳賢  新潟大学, 医歯学系, 講師 (80444157)
Research Collaborator SATO Yuya  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsネフローゼ症候群 / iPS細胞 / 糸球体上皮細胞 / プロラクチン / 性差 / 遺伝性ネフローゼ症候群 / Tリンパ球 / T細胞 / 初期化遺伝子 / FSGS / MCNS
Outline of Final Research Achievements

The purpose of this study was to establish iPS cells from peripheral T-cells of healthy volunteers or patients with hereditary nephrotic syndrome, and to introduce differentiation into glomerular podocyte,and investigate their functions. We succeeded to establish the iPS cells and found that the differentiated cells from these iPSs were positive with nephrin, podocin, synaptopodin, podocalyxin. However, any functional difference was not observed on cells originated from patients with hereditary nephrotic syndrome as compared to those from healthy controls, in respect with the response to renin-angiotensin, inflammatory cytokines, etc. In parallel, we also demonstrated that male-predominant Mups expression and MUPs production and urine excretion were down-regulated, whereas female-predominant genes such as Cyp3a41 and Cyp3a44 expressions were up-regulated by prolactin, and that the regulatory role of prolactin was independent with sex-specific GH secretion pattern or testosterone.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (42 results)

All 2017 2016 2015 2014

All Journal Article (18 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 18 results,  Acknowledgement Compliant: 7 results,  Open Access: 10 results) Presentation (14 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Book (10 results)

  • [Journal Article] Prolactin up-regulates female-predominant Cyp gene expressions and down-regulates male-predominant gene expressions in mice liver2017

    • Author(s)
      Sato Y, Kaneko Y, Cho T, Goto K, Otsuka T, Yamamoto S, Goto S, Maruyama H, Narita I.
    • Journal Title

      Drug Metab Dispos

      Volume: in press Issue: 6 Pages: 586-592

    • DOI

      10.1124/dmd.116.074658

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Megalin Blockade with Cilastatin Suppresses Drug-Induced Nephrotoxicity2017

    • Author(s)
      Hori Y, Aoki N, Kuwahara S, Hosojima M, Kaseda R, Goto S, Iida T, De S, Kabasawa H, Kaneko R, Aoki H, Tanabe Y, Kagamu H, Narita I, Kikuchi T, Saito A.
    • Journal Title

      J Am Soc Nephrol

      Volume: in press Issue: 6 Pages: 1783-1791

    • DOI

      10.1681/asn.2016060606

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exocytosis-Mediated Urinary Full-Length Megalin Excretion is Linked with the Pathogenesis of Diabetic Nephropathy2017

    • Author(s)
      De S, Kuwahara S, Hosojima M, Ishikawa T, Kaseda R, Sarkar P, Yoshioka Y, Kabasawa H, Iida T, Goto S, Toba K, Higuchi Y, Suzuki Y, Hara M, Kurosawa H, Narita I, Hirayama Y, Ochiya T, Saito A
    • Journal Title

      Diabetes

      Volume: in press Issue: 5 Pages: 1391-1404

    • DOI

      10.2337/db16-1031

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible role for glomerular-derived angiotensinogen in nephrotic syndrome.2017

    • Author(s)
      Yamazaki M, Fukusumi Y, Kayaba M, Kitazawa Y, Takamura S, Narita I, Kawachi H
    • Journal Title

      J Renin Angiotensin Aldosterone Syst

      Volume: in press Issue: 4

    • DOI

      10.1177/1470320316681223

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy2017

    • Author(s)
      Watanabe H, Goto S, Mori H, Higashi K, Hosomichi K, Aizawa N, Takahashi N, Tsuchida M, Suzuki Y, Yamada T, Horii A, Inoue I, Kurokawa K, Narita I.
    • Journal Title

      Nephrol Dial Transplant

      Volume: in press Pages: 2072-2079

    • DOI

      10.1093/ndt/gfw343

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oral pharmacological chaperone migalastat compared with enzyme replacement therapy in Fabry disease: 18-month results from the randomised phase III ATTRACT study2017

    • Author(s)
      Hughes DA, Nicholls K, Shankar SP, Ohashi T, Olivotto I, Sakai N, Eyskens F, Germain DP, Goker-Alpan O, Hachulla E, Jovanovic A, Lourenco CM, Narita I, Thomas M,
    • Journal Title

      J Med Genet

      Volume: 54 Issue: 4 Pages: 288-296

    • DOI

      10.1136/jmedgenet-2016-104178

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Extracapillary proliferation and arteriolar hyalinosis are associated with long-term kidney survival in IgA nephropathy2016

    • Author(s)
      Kaneko Y, Yoshita K, Kono E, Ito Y, Imai N, Yamamoto S, Goto S, Narita I.
    • Journal Title

      Clin Exp Nephrol

      Volume: in press Issue: 4 Pages: 569-577

    • DOI

      10.1007/s10157-015-1185-0

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Megalin-Mediated Tubuloglomerular Alterations in High-Fat Diet-Induced Kidney Disease2016

    • Author(s)
      Kuwahara S, Hosojima M, Kaneko R, Aoki H, Nakano D, Sasagawa T, Kabasawa H, Kaseda R, Yasukawa R, Ishikawa T, Suzuki A, Sato H, Kageyama S, Tanaka T, Kitamura N, Narita I, Komatsu M, Nishiyama A, Saito A
    • Journal Title

      J Am Soc Nephro

      Volume: in press Issue: 7 Pages: 1996-2008

    • DOI

      10.1681/asn.2015020190

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Administration of ferric citrate hydrate decreases circulating FGF23 levels independently of serum phosphate levels in hemodialysis patients with iron deficiency2016

    • Author(s)
      Akira Iguchi, Junichiro J. Kazama, Kazuhiro Yoshita, Yasuo Watanabe, Noriaki Iino, Ichiei Narita
    • Journal Title

      Nephron

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Leptin deficiency down-regulates IL-23 production in glomerular podocytes resulting in an attenuated immune response in nephrotoxic serum nephritis.2015

    • Author(s)
      Goto K, Kaneko Y, Sato Y, Otsuka T, Yamamoto S, Goto S, Yamamoto K, Yamamoto T, Kawachi H, Madaio MP, Narita I
    • Journal Title

      Int Immunol

      Volume: 28 Issue: 4 Pages: 197-208

    • DOI

      10.1093/intimm/dxv067

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate.2015

    • Author(s)
      Matsuo K, Yamamoto S, Wakamatsu T, Takahashi Y, Kawamura K, Kaneko Y, Goto S, Kazama JJ, Narita I.
    • Journal Title

      Toxins

      Volume: 7 Issue: 8 Pages: 3155-3166

    • DOI

      10.3390/toxins7083155

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Continuous Reduction of Protein-Bound Uraemic Toxins with Improved Oxidative Stress by Using the Oral Charcoal Adsorbent AST-120 in Haemodialysis Patients.2015

    • Author(s)
      Yamamoto S, Kazama JJ, Omori K, Matsuo K, Takahashi Y, Kawamura K, Matsuto T, Watanabe H, Maruyama T, Narita I.
    • Journal Title

      Sci Rep.

      Volume: N/A Issue: 1 Pages: 14381-14381

    • DOI

      10.1038/srep14381

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Atherosclerosis following renal injury is ameliorated by pioglitazone and losartan via macrophage phenotype.2015

    • Author(s)
      Yamamoto S, Zhong J, Yancey PG, Zuo Y, Linton MF, Fazio S, Yang H, Narita I, Kon V.
    • Journal Title

      Atherosclerosis

      Volume: 242 Issue: 1 Pages: 56-64

    • DOI

      10.1016/j.atherosclerosis.2015.06.055

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Specific overexpression of TNFAIP9 in CD14 CD16 monocytes in patients with rheumatoid arthritis:Comparative analysis with TNFAIP32015

    • Author(s)
      Takai C, Matsumoto I, Inoue A, Umeda N, Tanaka Y, Kurashima Y, Wada Y, Narita I, Sumida T
    • Journal Title

      Clin Exp Immuno

      Volume: 印刷中 Issue: 3 Pages: 458-466

    • DOI

      10.1111/cei.12606

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Use of Renin-Angiotensin System Inhibitors Is Associated with Reduction of Fracture Risk in Hemodialysis Patients2015

    • Author(s)
      Yamamoto S, Kido R, Ohashi Y, Fukuma S, Akizawa T, Fukagawa M, Kazama J, Narita I.
    • Journal Title

      PloS one 201

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of the p.E66Q variant of GLA in the progression of chronic kidney disease2014

    • Author(s)
      Watanabe H, Goto S, Miyashita A, Maruyama H, Wakasugi M, Yokoseki A, Kuwano R, Narita I
    • Journal Title

      Clin Exp Nephrol

      Volume: Epub ahead of print Issue: 2 Pages: 225-230

    • DOI

      10.1007/s10157-014-0969-y

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Upregulation of prolactin receptor in proximal tubular cells was induced in cardiac dysfunction model mice.2014

    • Author(s)
      Tsuchida Y, Kaneko Y, Otsuka T, Goto K, Saito A, Yamamoto K, Yamamoto T, Narita I
    • Journal Title

      Clinical and Experimental Nephrology

      Volume: 18 Issue: 1 Pages: 65-74

    • DOI

      10.1007/s10157-013-0820-x

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Discovery of new risk loci for IgA nephropathy implicates genes involved in immunity against intestinal pathogens.2014

    • Author(s)
      Kiryluk K, , Suzuki H, Wyatt RJ, Novak J, Julian BA, Feehally J, Lifton RP, Gharavi AG, et al.
    • Journal Title

      Nat Genet

      Volume: 46 Issue: 11 Pages: 1187-1196

    • DOI

      10.1038/ng.3118

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Two Forms of Urinary Megalin Excretion Are Novel Predictors of the Progression of Early-Stage Diabetic Nephropathy in Type 2 Diabetes Mellitus.2016

    • Author(s)
      Iida T, Hosojima M, Kabasawa K, Nakamura K, Kuwahara S, Ishikawa T, Kaseda R, Suzuki Y, Narita I, Saito A.
    • Organizer
      American Society of Nephrology 2016
    • Place of Presentation
      Convention Center, Chicago, IL, USA
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Protein-Bound Uremic Toxin Adsorption by Hexadecyl-Immobilized Cellulose Beads in Hemodialysis.2016

    • Author(s)
      Yamamoto S, Sato M, Wakamatsu T, Takahashi Y, Iguchi A, Omori K, Suzuki Y, Ei I, Kazama J, Gejyo F, Narita I
    • Organizer
      American Society of Nephrology 2016,
    • Place of Presentation
      Convention Center, Chicago, IL, USA
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Differential Effects of Angiotensin II Receptor Blocker (ARB) versus ACE Inhibitor (ACEI) on HDL Functionality in Patients on Maintenance Hemodialysis (MHD).2016

    • Author(s)
      Kaseda R, Tsuchida Y, Zhong J, Narita I, Ikizler T, Kon V
    • Organizer
      American Society of Nephrology 2016
    • Place of Presentation
      Convention Center, Chicago, IL, USA
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Renin-Angiotensin System Inhibition Ameliorates Bone Fragility Through Blocking Both Osteocytic and Osteoblastic Actions in Uremic Rats.2016

    • Author(s)
      Wakamatsu T, Iwasaki Y, Yamamoto S, Ito A, Takahashi Y, Gejyo F, Kazaa J, Fukagawa M, Narita I
    • Organizer
      American Society of Nephrology 2016
    • Place of Presentation
      Convention Center, Chicago, IL, USA
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] (シンポジウム)IgA腎症の口蓋扁桃陰窩のマイクロバイオーム解析2016

    • Author(s)
      後藤 眞、渡辺博文、土田雅史、井ノ上逸朗、黒川 顕、成田一衛
    • Organizer
      第59回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜、神奈川県、横浜市
    • Year and Date
      2016-06-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comprehensive Microbiome Analysis of Tonsillar Crypts in IgA Nephropathy2015

    • Author(s)
      Hirofumi Watanabe, Shin Goto, Masafumi Tsuchida, Ichiei Narita
    • Organizer
      American Society of Nephrology Kidney Week 2015
    • Place of Presentation
      Convention Center, San Diego, USA
    • Year and Date
      2015-11-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanism of Increased Urinary Full-Length Megalin Excretion in Type 2 Diabetes Mellitus Patients with Nephropathy2015

    • Author(s)
      Shankhajit De, Shoji Kuwahara, Michihiro Hosojima, Tomomi Ishikawa, Ryohei Kaseda, Yusuke Yoshioka, Yoshiki Suzuki, Ichiei Narita, Takahiro Ochiya, Akihiko Saito
    • Organizer
      American Society of Nephrology Kidney Week 2015
    • Place of Presentation
      Convention Center, San Diego, USA
    • Year and Date
      2015-11-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ワークショップ 尿毒素物質の除去と患者予後2015

    • Author(s)
      山本  卓、風間 順一郎、成田 一衛
    • Organizer
      第60回日本透析医学会学術集会・総会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 総会長主導企画 IgA腎症の責任遺伝子探索―候補遺伝子解析から疾患感受性は予測可能か―2015

    • Author(s)
      成田 一衛、後藤  眞
    • Organizer
      第58回日本腎臓学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県、名古屋市)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 家族性非典型溶血性尿毒症症候群のエクソーム解析2015

    • Author(s)
      渡辺 博文、後藤 眞、山崎 肇、土田 雅史 、日高 義彦 、渡部 登志雄 、山本 格、成田 一衛
    • Organizer
      第58回日本腎臓学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県、名古屋市)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report
  • [Presentation] Exome Sequencing Identified Novel Mutations of FKRP in Familial Atypical Hemolytic Uremic Syndrome2014

    • Author(s)
      Watanabe H, Goto S, Yamazaki H, Narita I.
    • Organizer
      American Society of Nephrology Kidney Week 2014
    • Place of Presentation
      Pennsylvania Convention Center, Philadelphia, PA, USA
    • Year and Date
      2014-11-11 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Leptin Deficiency Inhibits IL-23/Th-17axis and Monocyte Chemoattractant Protein-1 Production in a Murine Model of Nephrotoxic Serum Nephritis2014

    • Author(s)
      Goto K, Kaneko Y, Goto S, Narita I
    • Organizer
      American Society of Nephrology Kidney Week 2014
    • Place of Presentation
      Pennsylvania Convention Center, Philadelphia, PA, USA
    • Year and Date
      2014-11-11 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Pathological Prognosticators and Therapeutic Impact of Tonsillectomy in IgA Nephropathy2014

    • Author(s)
      Kaneko Y, Goto S, Narita I
    • Organizer
      American Society of Nephrology Kidney Week 2014
    • Place of Presentation
      Pennsylvania Convention Center, Philadelphia, PA, USA
    • Year and Date
      2014-11-11 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 総会長主導企画 :5つの健康習慣(禁煙,体重管理, 節酒,身体活動,食事)の効果2014

    • Author(s)
      若杉三奈子、風間順一郎、成田 一衛
    • Organizer
      第57回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜、横浜市、神奈川県
    • Year and Date
      2014-07-04 – 2014-07-06
    • Related Report
      2014 Research-status Report
  • [Book] IgA 腎症の発症因子(遺伝要因):腎疾患・透析最新の治療2017-20192017

    • Author(s)
      後藤 眞、 成田 一衛
    • Publisher
      南江堂
    • Related Report
      2016 Annual Research Report
  • [Book] 薬剤性腎障害に関する最近の話題:Annual Review腎臓 20162016

    • Author(s)
      酒巻 裕一、成田 一衛
    • Total Pages
      205
    • Publisher
      中外医学社・東京
    • Related Report
      2016 Annual Research Report
  • [Book] 透析関連アミロイドーシスの治療 update: 腎と骨~ CKD-MBDの概念から新たな展開へ~2016

    • Author(s)
      山本  卓、成田 一衛
    • Total Pages
      231
    • Publisher
      医薬ジャーナル社・大阪
    • Related Report
      2016 Annual Research Report
  • [Book] 多発性嚢胞腎(PKD): year note 第27版2016

    • Author(s)
      成田 一衛
    • Total Pages
      471
    • Publisher
      メディックメディア
    • Related Report
      2016 Annual Research Report
  • [Book] Is IgAN a familial or sporadic disease? in Pathogenesis and Treatment in IgA Nephropathy, Tomino Y, ed.2016

    • Author(s)
      Narita I, Kaneko Y, Itho Y, Sakamaki Y, Iguchi S, Yamamoto S, Wakasugi M, Kazama JJ, Goto S.
    • Publisher
      Springer
    • Related Report
      2015 Research-status Report
  • [Book] 薬剤性腎障害に関する最近の話題:Annual Review腎臓20162016

    • Author(s)
      酒巻裕一、成田一衛
    • Total Pages
      205
    • Publisher
      中外医学社
    • Related Report
      2015 Research-status Report
  • [Book] 腎臓病学におけるゲノミクス:腎臓内科・泌尿器科2015

    • Author(s)
      土田雅史、後藤 眞、成田一衛
    • Total Pages
      310
    • Publisher
      科学評論社
    • Related Report
      2015 Research-status Report
  • [Book] 腎移植後再発性腎炎とそのメカニズム2015

    • Author(s)
      酒巻裕一、成田一衛
    • Total Pages
      194
    • Publisher
      科学評論社
    • Related Report
      2015 Research-status Report
  • [Book] マイクロバイオーム研究の展望. Annual Review腎臓 20142014

    • Author(s)
      渡部博文、後藤眞、成田一衛
    • Total Pages
      230
    • Publisher
      中外医学社
    • Related Report
      2014 Research-status Report
  • [Book] 腎臓病・高血圧と薬剤 実践Q&A2014

    • Author(s)
      河野恵美子、成田一衛
    • Total Pages
      246
    • Publisher
      中外医学社
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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