Reproduction of podocyte tertiary structure under cultured condition
Project/Area Number |
26670431
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Kidney internal medicine
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Research Institution | Kyoto University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
SHIRATA NARITOSHI 京都大学, 大学院医学研究科, 客員研究員 (00726200)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ポドサイト / 糸球体硬化 / Dendrin / MAGI-2 / Podocin / SNX-9 / スリット膜 / 足突起 / SNX9 / 蛋白分解 |
Outline of Final Research Achievements |
It has recently become clear that initial glomerular injury affects glomerular podocytes, making them important target cells for progression of chronic kidney disease (CKD). Podocyte injury may cause podocyte detachment from the GBM, which leads to glomerulosclerosis. We have reported that dendrin translocates to the nucleus in injured podocytes, promoting podocyte apoptosis. Moreover, nuclear translocation of dendrin was also found in human kidney biopsy specimens from patients with glomerular diseases characterized by podocyte loss and glomerulosclerosis. We found that MAGI-2 is necessary for inhibiting dendrin nuclear translocation under physiological conditions. We have demonstrated the endocytic translocation of podocin to the subcellular area in injured podocytes. we identified SNX9 as a facilitator of podocin endocytosis in severe podocyte injury and demonstrated the expression of SNX9 in the podocytes of irreversible glomerular disease.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] UPS40 gene knockdown disrupts glomerular permwability in zebrafish.2017
Author(s)
Takagi H, Nishibori Y, Katayama K, Katada T, Takahashi S, Kikuchi Z, Takahashi SI, Kamei H, Kawakami H, Akimoto Y, Kudo A, Asanuma K, Takematsu H, Yan K.
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Journal Title
Am J Physiol Renal Physiol.
Volume: 312
Issue: 4
Pages: 702-715
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Sorting Nexin 9 facilitates podocin endocytosis in the injured podocyte.2017
Author(s)
Sasaki Y, Hidaka T, Ueno T, Akiba-Takagi M, Trejo JA, Seki T, Nagai-Hosoe Y, Tanaka E, Horikoshi S, Tomino Y, Suzuki Y, Asanuma K.
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Journal Title
10.1038/srep43921.2017
Volume: 7
Issue: 1
Pages: 43921-43921
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Impaired podocyte autophagy exacerbates proteinuria in diabetic nephropathy.2016
Author(s)
Tagawa A, Yasuda M, Kume S, Ymahara K, Nakazawa J, Chin-Kanasaki M, Araki H, Araki SI, Koya D, Asanuma K, Kim EH, Haneda M, Kajiwara N, Hayashi K, Ohashi H, Ugi S, Maegawa H, Uzu T
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Journal Title
Diabetes.
Volume: -
Issue: 3
Pages: 755-767
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Cathepsin D in podocytes plays an important role in the pathogenesis of proteinuria and chronic kidney disease.2016
Author(s)
Nonaka-Yamamoto K, Koike M, Asanuma K, Akiba-Takagi M, Trejo JAO, Seki T, Hidaka T, Ichimura K, Sakai T, Tada N, Ueno T, Uchiyama Y, Tomino Y.
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Journal Title
J Am Soc Nephrol
Volume: -
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Podocyte-specific deletion of Rac1 leads to aggravation of renal injury in STZ-induced diabetic mice2015
Author(s)
Masanori Ishizaka, Tomohito Gohda, Miyuki Takagi, Keisuke Omote, Yuji Sonoda, Juan Alejandro Oliva Trejo, Rin Asao, Teruo Hidaka, Katsuhiko Asanuma, Satoshi Horikoshi, Yasuhiko Tomino
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 467
Issue: 3
Pages: 549-555
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] 尿中メガリンと糸球体病変との関連.2015
Author(s)
関 卓人, 淺沼 克彦, 浅尾 りん, 野中 香苗, 佐々木 有, 黒澤 寛之, 平山 吉朗, 富野 康日己.
Organizer
第112回日本内科学会講演会
Place of Presentation
京都
Year and Date
2015-04-11
Related Report