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Reproduction of podocyte tertiary structure under cultured condition

Research Project

Project/Area Number 26670431
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Kidney internal medicine
Research InstitutionKyoto University

Principal Investigator

ASANUMA KATSUHIKO  京都大学, 医学研究科, 特定准教授 (60449064)

Co-Investigator(Renkei-kenkyūsha) SHIRATA NARITOSHI  京都大学, 大学院医学研究科, 客員研究員 (00726200)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsポドサイト / 糸球体硬化 / Dendrin / MAGI-2 / Podocin / SNX-9 / スリット膜 / 足突起 / SNX9 / 蛋白分解
Outline of Final Research Achievements

It has recently become clear that initial glomerular injury affects glomerular podocytes, making them important target cells for progression of chronic kidney disease (CKD). Podocyte injury may cause podocyte detachment from the GBM, which leads to glomerulosclerosis. We have reported that dendrin translocates to the nucleus in injured podocytes, promoting podocyte apoptosis. Moreover, nuclear translocation of dendrin was also found in human kidney biopsy specimens from patients with glomerular diseases characterized by podocyte loss and glomerulosclerosis. We found that MAGI-2 is necessary for inhibiting dendrin nuclear translocation under physiological conditions. We have demonstrated the endocytic translocation of podocin to the subcellular area in injured podocytes. we identified SNX9 as a facilitator of podocin endocytosis in severe podocyte injury and demonstrated the expression of SNX9 in the podocytes of irreversible glomerular disease.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014

All Journal Article (13 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 11 results,  Open Access: 6 results,  Acknowledgement Compliant: 6 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] UPS40 gene knockdown disrupts glomerular permwability in zebrafish.2017

    • Author(s)
      Takagi H, Nishibori Y, Katayama K, Katada T, Takahashi S, Kikuchi Z, Takahashi SI, Kamei H, Kawakami H, Akimoto Y, Kudo A, Asanuma K, Takematsu H, Yan K.
    • Journal Title

      Am J Physiol Renal Physiol.

      Volume: 312 Issue: 4 Pages: 702-715

    • DOI

      10.1152/ajprenal.00197.2016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Sorting Nexin 9 facilitates podocin endocytosis in the injured podocyte.2017

    • Author(s)
      Sasaki Y, Hidaka T, Ueno T, Akiba-Takagi M, Trejo JA, Seki T, Nagai-Hosoe Y, Tanaka E, Horikoshi S, Tomino Y, Suzuki Y, Asanuma K.
    • Journal Title

      10.1038/srep43921.2017

      Volume: 7 Issue: 1 Pages: 43921-43921

    • DOI

      10.1038/srep43921

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The role of Notch signaling in kidney podocytes.2017

    • Author(s)
      Asanuma K, Trejo JAO, Tanaka E,
    • Journal Title

      Clin Exp Nephrol.

      Volume: 21 Pages: 1-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Establishment of Nephrin Reporter Mice and Use for Chemical Screening.2016

    • Author(s)
      Tsuchida K, Matsusaka T, Ohtcuka M, Miura H, Okuno Y, Asanuma K, Nakagawa T, Yanagita M, Mori K.
    • Journal Title

      PLoS One.

      Volume: 11 Issue: 6 Pages: 2699-2708

    • DOI

      10.1371/journal.pone.0157497

    • NAID

      120005825910

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impaired podocyte autophagy exacerbates proteinuria in diabetic nephropathy.2016

    • Author(s)
      Tagawa A, Yasuda M, Kume S, Ymahara K, Nakazawa J, Chin-Kanasaki M, Araki H, Araki SI, Koya D, Asanuma K, Kim EH, Haneda M, Kajiwara N, Hayashi K, Ohashi H, Ugi S, Maegawa H, Uzu T
    • Journal Title

      Diabetes.

      Volume: - Issue: 3 Pages: 755-767

    • DOI

      10.2337/db15-0473

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cathepsin D in podocytes plays an important role in the pathogenesis of proteinuria and chronic kidney disease.2016

    • Author(s)
      Nonaka-Yamamoto K, Koike M, Asanuma K, Akiba-Takagi M, Trejo JAO, Seki T, Hidaka T, Ichimura K, Sakai T, Tada N, Ueno T, Uchiyama Y, Tomino Y.
    • Journal Title

      J Am Soc Nephrol

      Volume: -

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Combination therapy with telmisartan and oxacalcitriol suppresses the progression of murine adriamycin nephropathy.2015

    • Author(s)
      Jeong KH, Asanuma K, Lydia A, Takagi M, Asao R, Kodama F, Asanuma E, Tomino Y.
    • Journal Title

      Nephron

      Volume: 129(2) Issue: 2 Pages: 143-154

    • DOI

      10.1159/000369346

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ENOS deficiency causes podocyte injury with mitochondrial abnormality2015

    • Author(s)
      Ueda S, Ozawa S, Mori K, Asanuma K, Yanagita M, Uchida S, Nakagawa T
    • Journal Title

      Free Radic Biol Med.

      Volume: 87 Pages: 181-192

    • DOI

      10.1016/j.freeradbiomed.2015.06.028

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Podocyte-specific deletion of Rac1 leads to aggravation of renal injury in STZ-induced diabetic mice2015

    • Author(s)
      Masanori Ishizaka, Tomohito Gohda, Miyuki Takagi, Keisuke Omote, Yuji Sonoda, Juan Alejandro Oliva Trejo, Rin Asao, Teruo Hidaka, Katsuhiko Asanuma, Satoshi Horikoshi, Yasuhiko Tomino
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 467 Issue: 3 Pages: 549-555

    • DOI

      10.1016/j.bbrc.2015.09.158

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Glycolysis, but not Mitocohdria, responsible for intracellular ATP distribution in cortical area of podocytes.2015

    • Author(s)
      Ozawa S, Ueda S, Imamura H, Mori K, Asanuma K, Yanagita M, Nakagawa T
    • Journal Title

      Sci Rep.

      Volume: 18 Pages: 18575-18575

    • Related Report
      2015 Research-status Report
  • [Journal Article] Podocin is translocated to cytoplasm in puromycin aminonucleoside nephrosis rats and in poor-prognosis patients with IgA nephropathy2015

    • Author(s)
      Fukuda H, Hidaka T, Takagi-Akiba M, Ichimura K, Trejo JA, Sasaki Y, Wang J, Sakai T, Asanuma K, Tomino Y.
    • Journal Title

      Cell Tissue Res.

      Volume: 360 Pages: 391-400

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The role of podocyte injury in chronic kidney disease2015

    • Author(s)
      Asanuma K.
    • Journal Title

      Japanese Journal of Clinical Immunology

      Volume: 38 Issue: 1 Pages: 26-36

    • DOI

      10.2177/jsci.38.26

    • NAID

      130004875949

    • ISSN
      0911-4300, 1349-7413
    • Related Report
      2014 Research-status Report
    • Open Access
  • [Journal Article] MAGI-2 is critical for the formation and maintenance of the glomerular filtration barrier in mouse kidney.2014

    • Author(s)
      Ihara K, Asanuma K, Fukuda T, Ohwada S, Yoshida M, Nishimori K
    • Journal Title

      The American journal of pathology

      Volume: 184 Issue: 10 Pages: 2699-2708

    • DOI

      10.1016/j.ajpath.2014.06.019

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] ポドサイトにおけるMAGI-2の機能.2017

    • Author(s)
      淺沼克彦, 伊原寛一郎, 西森克彦, 柳田素子.
    • Organizer
      第1回ポドサイト研究会
    • Place of Presentation
      新潟
    • Year and Date
      2017-03-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] Mechanism of the progression from podocyte injury to glomerulosclerosis in chronic kidney disease2017

    • Author(s)
      Katsuhiko Asanuma
    • Organizer
      The 2017 Japan-NIH Joint Symposium
    • Place of Presentation
      Sendai
    • Year and Date
      2017-02-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Podocyte-specific MAGI-2 deficiency results in nephrosis and renal failure.2017

    • Author(s)
      Naritoshi Shirata, Kan-ichiro Ihara, Motoko Yanagita, Katsuhiko Nishimori, Katsuhiko Asanuma.
    • Organizer
      The 2017 Japan-NIH Joint Symposium
    • Place of Presentation
      新潟
    • Year and Date
      2017-02-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 腎糸球体上皮細胞(ポドサイト)障害促進因子dendrinの核移行メカニズムの解明2016

    • Author(s)
      白田成俊,伊原寛一郎,柳田素子,西森克彦,淺沼克彦
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] The mechanisms of dendrin nuclear translocation in kidney podocytes.2016

    • Author(s)
      Naritoshi Shirata, Kan-ichiro Ihara, Motoko Yanagita, Katsuhiko Nishimori, Katsuhiko Asanuma.
    • Organizer
      ASN KIDNEY WEEK 2016
    • Place of Presentation
      Chicago
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ポドサイト障害促進因子dendrinの核移行メカニズムの解明2016

    • Author(s)
      白田成俊,伊原寛一郎,柳田素子,西森克彦,淺沼克彦
    • Organizer
      第59回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2016-06-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] ポドサイトにおけるカテプシンDの病態生理的役割.第59回日本腎臓学会学術総会2016

    • Author(s)
      山本香苗,淺沼克彦,小池正人,高木美幸,Oliva Trejo JA, 関卓人,日高輝夫,市村浩一郎,坂井建雄,上野隆,内山安男,富野康日己
    • Organizer
      第59回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2016-06-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] TelmisartanとOxacalcitriolの組み合わせ投与は、マウスアドリアマイシン腎症の進行を抑制する.2015

    • Author(s)
      淺沼 克彦, Jeong Kyoung Hwan, Lydia Aida, 高木 美幸, 浅尾 りん, 淺沼 悦子, 富野 康日己.
    • Organizer
      第38回日本高血圧学会総会
    • Place of Presentation
      松山
    • Year and Date
      2015-10-10
    • Related Report
      2015 Research-status Report
  • [Presentation] ネフローゼ症候群の分子メカニズム2015

    • Author(s)
      淺沼克彦
    • Organizer
      第45回日本腎臓学会東部学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] RGS and FOSL-1 were identified molecules related to podocyte injury induced by Adriamycin.2015

    • Author(s)
      Wan Juan, 淺沼 克彦, 日高 輝夫, 佐々木 有, 田中 絵里子, 高木-秋葉 美幸, 富野 康日己.
    • Organizer
      第58回日本腎臓学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
  • [Presentation] ピューロマイシン腎症ラットとIgA腎症予後不両軍におけるポドシンの局在変化.2015

    • Author(s)
      佐々木 有, 福田 裕光, 日高 輝夫, 淺沼 克彦, 市村 浩一郎, 坂井 建雄, 富野 康日己.
    • Organizer
      第58回日本腎臓学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-06-06
    • Related Report
      2015 Research-status Report
  • [Presentation] シンポジウム2腎臓領域の基礎研究の現在と未来2015

    • Author(s)
      淺沼克彦
    • Organizer
      第58回日本腎臓学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-06-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 尿中メガリンと糸球体病変との関連.2015

    • Author(s)
      関 卓人, 淺沼 克彦, 浅尾 りん, 野中 香苗, 佐々木 有, 黒澤 寛之, 平山 吉朗, 富野 康日己.
    • Organizer
      第112回日本内科学会講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-04-11
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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