Analysis of RNA binding proteins in polyglutamine disease
Project/Area Number |
26670445
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurology
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Research Institution | Yokohama City University |
Principal Investigator |
TANAKA Fumiaki 横浜市立大学, 医学(系)研究科(研究院), 教授 (30378012)
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Co-Investigator(Kenkyū-buntansha) |
DOI Hiroshi 横浜市立大学, 医学部, 講師 (10326035)
KOYANO Shigeru 横浜市立大学, 医学部, 准教授 (50315818)
TADA Mikiko 横浜市立大学, 附属病院, 助教 (30722467)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | ポリグルタミン病 / PCBP / RNA結合タンパク / 核内封入体 |
Outline of Final Research Achievements |
Using aggregate proteome analysis we identified many component proteins of polyglutamine nuclear aggregates including RNA binding proteins. In this study we analyzed the staining patterns of PCBP1, PCBP2, PCBP3, hnRNPU, hnRNP H1, hnRNP H2, hnRNP F, DDX5, DDX17, Matrin 3, and SGTA in human autopsy brains of various polyglutamine diseases. As a result, Matrin 3 and SGTA were observed in nuclear aggregates. Preliminary result shows that SGTA suppresses aggregate formation in Huntington's disease cell model. Further, we identified SGTA-binding proteins using mass spectrometry.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Next-generation sequencing of 28 ALS-related genes in a Japanese ALS cohort2016
Author(s)
Ryoichi Nakamura, Jun Sone, Naoki Atsuta, Genki Tohnai, Hazuki Watanabe, Daichi Yokoi, Masahiro Nakatochi, Hirohisa Watanabe, Mizuki Ito, Jo Senda, Masahisa Katsuno, Fumiaki Tanaka, Yuanzhe Li, Yuishin Izumi, Mitsuya Morita, Akira Taniguchi, Osamu Kano, Masaya Oda, Satoshi Kuwabara, Koji Abe.
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Journal Title
Neurobiology of Aging
Volume: 39
Pages: 219-219
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A Japanese case of cerebellar ataxia, spastic paraparesis and deep sensory impairment associated with a novel homozygous TTC19 mutation2015
Author(s)
Kunii M, Doi H, Higashiyama Y, Kugimoto C, Ueda N, Hirata J, Tomita-Katsumoto A, Kashikura-Kojima M, Kubota S, Taniguchi M, Murayama K, Nakashima M, Tsurusaki Y, Miyake N, Saitsu H, Matsumoto N, Tanaka F.
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Journal Title
J Hum Genet
Volume: 60
Issue: 4
Pages: 187-191
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Late-onset spastic ataxia phenotype in a patient with a homozygous DDHD2 mutation2014
Author(s)
Doi H, Ushiyama M, Baba T, Tani K, Shiina M, Ogata K, Miyatake S, Yuzawa YF, Tsuji S, Nakashima M, Tsurusaki Y, Miyake N, Saitsu H, Ikeda S, Tanaka F, Matsumoto N, Yoshida K
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Journal Title
Sci Rep
Volume: 4
Issue: 1
Pages: 7132-7132
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Schwann cell involvement in the peripheral neuropathy of spinocerebellar ataxia type 3.2014
Author(s)
Suga N, Katsuno M, Koike H, Banno H, Suzuki K, Hashizume A, Mano T, Iijima M, Kawagashira Y, Hirayama M, Nakamura T, Watanabe H, Tanaka F, Sobue G
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Journal Title
Neuropathol Appl Neurobiol.
Volume: 40
Issue: 5
Pages: 628-639
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Analysis of major amyotrophic lateral sclerosis genes in Japan2014
Author(s)
Nakamura R, Sone J, Atsuta N, Watanabe H, Hirakawa A, Watanabe H, Ito M, Senda J, Katsuno K, Tanaka F, Izumi Y, Morita M, Ogaki K, Taniguchi A, Aiba A, Mizoguchi K, Okamoto K, Hasegawa K, Aoki M, Kawata A, Abe K, Oda M, Konagaya M, Imai T, Nakagawa M, Tsuji S, Kaji R, Nakano I, Sobue G
Organizer
64th Annual Meeting of the American Society of Human Genetics
Place of Presentation
San Diego Convention Center, San Diego, CA, USA
Year and Date
2014-10-18 – 2014-10-22
Related Report