A novel pathway of the differenciation of Beige cells - analisis of adipocyte specific EID1-overexpressed mice
| Project/Area Number |
26670457
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Gunma University |
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Principal Investigator |
Koibuchi Noriyuki 群馬大学, 医学(系)研究科(研究院), 教授 (80234681)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Toshiharu 神奈川県立保健福祉大学, 保健福祉学部, 人間総合・専門基礎担当・教授 (80375576)
SHIMOKAWA Noriaki 高崎健康福祉大学, 健康福祉学部・健康栄養学科, 教授 (90235680)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 褐色脂肪 / EID1 / トランスジェニックマウス / ベージュ細胞 |
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| Outline of Final Research Achievements |
To investigate the function of EID1 in the adipogenesis in vivo, we generated mice overexpressed EID1 only adipose tissues (subcutaneous, visceral and brown adipose tissues). We confirmed a successful integration of the expression vector into the genome of mice by PCR. However, we could not furthermore confirm the protein expression of EID1 in adipose tissues by western blot analysis. We are currently trying a variety of approaches to protein expression of EID1 in the transgenic mice, including cold exposure, intake of high-fat diet and administration of PPARγ agonist such as Rosiglitazone. Moreover, we are analyzing the glucose kinetics in brown adipose tissue of EID1 transgenic mice after cold exposure by 18-Fluoro-deoxyglucose positron emission tomography (FDG-PET).
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Report
(3 results)
Research Products
(36 results)
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[Journal Article] Possible involvement of IGF-1 signaling on compensatory growth of the infraspinatus muscle induced by the supraspinatus tendon detachment of rat shoulder2014
Author(s)
Ichinose T, Lesmana R, Yamamoto A, Kobayashi T, Shitara H, Shimoyama D, Takatsuru Y, Iwasaki T, Shimokawa N, Takagishi K, Koibuchi N
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Journal Title
Physiological Rep
Volume: 2
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines.2014
Author(s)
Yamazaki H, Kojima N, Kato K, Hirose E, Iwasaki T, Mizui T, Takahashi H, Hanamura K, Roppongi RT, Koibuchi N, Sekino Y, Mori N, Shirao T.
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Journal Title
J Neurochem.
Volume: 128
Issue: 4
Pages: 507-522
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Altered cerebellum development and dopamine distribution in a rat genetic model with congenital hypothyroidism.2014
Author(s)
Shimokawa N, Yousefi B, Morioka S, Yamaguchi S, Ohsawa A, Hayashi H, Azuma A, Mizuno H, Kasagi M, Masuda H, Jingu H, Furudate SI, Haijima A, Takatsuru Y, Iwasaki T, Umezu M, Koibuchi N.
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Journal Title
J Neuroendocrinol.
Volume: 26
Issue: 3
Pages: 164-175
DOI
Related Report
Peer Reviewed / Open Access
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