Screening of factors regulating refractory leukemia using genome editting

• Project/Area Number: 26670464
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: KUROKAWA MINEO 東京大学, 医学部附属病院, 教授 (80312320)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: EVI1 / Genome editting / TALEN / Acute myeloid leukemia / 制御因子

Outline of Final Research Achievements

EVI1 transcription factor is a proto-oncogene which is associated with myeloid malignancy with poor prognosis. To search EVI1-up-regulating factors in a genome-wide scale, we made an acute myeloid leukemia cell line of which fluorescent reporter gene was inserted in EVI1 gene region. cDNA expression library derived from a cell line highly expressing EVI1 was virally transducted to the EVI1-reporter cell line and we isolated cells which exhibited stronger reporter signal. We examined the genome and obtained some candidate genes which may positively control EVI1. We are now closely investigating the function of these genes.

Research Products

• [Journal Article] Evi1 defines leukemia-initiating capacity and tyrosine kinase inhibitor resistance in chronic myeloid leukemia. (2014)
  • Author(s): Sato T, Goyama S, Kataoka K, Nasu R, Tsuruta-Kishino T, Kagoya Y, Nukina A, Kumagai K, Kubota N, Nakagawa M, Arai S, Yoshimi A, Honda H, Kadowaki T, Kurokawa M.
  • Journal Title: Oncogene Volume: 33 Issue: 42 Pages: 5028-5038
  • DOI: 10.1038/onc.2014.108
  • Peer Reviewed / Open Access / Acknowledgement Compliant