Screening of factors regulating refractory leukemia using genome editting

Research Project

Project/Area Number	26670464
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	KUROKAWA MINEO 東京大学, 医学部附属病院, 教授 (80312320)
Project Period (FY)	2014-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords	EVI1 / Genome editting / TALEN / Acute myeloid leukemia / 制御因子
Outline of Final Research Achievements	EVI1 transcription factor is a proto-oncogene which is associated with myeloid malignancy with poor prognosis. To search EVI1-up-regulating factors in a genome-wide scale, we made an acute myeloid leukemia cell line of which fluorescent reporter gene was inserted in EVI1 gene region. cDNA expression library derived from a cell line highly expressing EVI1 was virally transducted to the EVI1-reporter cell line and we isolated cells which exhibited stronger reporter signal. We examined the genome and obtained some candidate genes which may positively control EVI1. We are now closely investigating the function of these genes.

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