| Project/Area Number |
26670473
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Tohoku University |
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Principal Investigator |
Setsuya Aiba 東北大学, 医学(系)研究科(研究院), 教授 (80159269)
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| Co-Investigator(Kenkyū-buntansha) |
Ito YUMIKO 東北大学, 病院, 技術補佐員 (00375057)
Mizuashi MASATO 東北大学, 病院, 助教 (20400369)
Kimura YUTAKA 東北大学, 病院, 助教 (90375056)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 表皮 / 鉄代謝 / フェロポーチン / ヘムオキシゲナーゼ / 表皮細胞 / 角化 |
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| Outline of Final Research Achievements |
Using immunohistochemistry and mRNA quantification, we showed that molecules participating in iron import and storage are expressed in the lower epidermis, whereas those used for iron release from heme or iron transport are expressed in the upper epidermis. This expression pattern of iron metabolism molecules was confirmed using normal human epidermal keratinocytes during in vitro differentiation. We next demonstrated that reducing ferroportin expression in vitro by ferroportin-specific siRNAs or hepcidin significantly increased the intracellular iron content. Finally, we showed that the iron content of the epidermis and squames was significantly greater in FpnEpi-KO mice than in control mice, and that FpnEpi-KO exhibited decreased blood Hb concentration more rapidly than control mice on a low iron diet. The epidermis is equipped with a machinery by which intracellular iron in differentiated keratinocytes is excreted to the extracellular space before reaching the stratum corneum.
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