Fabrication of biological arterial graft for congenital heart disease

• Project/Area Number: 26670506
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Yokohama City University
• Principal Investigator: YOKOYAMA Utako 横浜市立大学, 医学部, 准教授 (70404994)
• Co-Investigator(Kenkyū-buntansha): 郷田 素彦 横浜市立大学, 附属病院, 講師 (30644570) ; 安田 章沢 横浜市立大学, 附属市民総合医療センター, 助教 (20468169) [Withdrawn]
• Co-Investigator(Renkei-kenkyūsha): MASUDA Munetaka 横浜市立大学, 医学研究科, 教授 (10190365) ; MATSUSAKI Michiya 大阪大学, 工学研究科, 助教 (00419467)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed(Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost : ¥2,800,000、Indirect Cost : ¥840,000); Fiscal Year 2016 : ¥780,000 (Direct Cost : ¥600,000、Indirect Cost : ¥180,000); Fiscal Year 2015 : ¥1,430,000 (Direct Cost : ¥1,100,000、Indirect Cost : ¥330,000); Fiscal Year 2014 : ¥1,430,000 (Direct Cost : ¥1,100,000、Indirect Cost : ¥330,000)
• Keywords: 再生医療 / 循環器・高血圧 / 平滑筋 / 人工血管

Outline of Final Research Achievements

We previously demonstrated that a layer by layer method using gelatin and fibronectin enabled to fabricate three-dimensional multi-layered smooth muscle cells. Based on this technique, we aimed to fabricate implantable arterial grafts. We optimized density of cell seeding and incubation time and obtained seven-layered multilayered smooth muscle cells that have 0.05 mm in thickness. The burst pressure was 1874±255 mmHg (718-3138 mmHg). We implanted the sheet into rat abdominal aorta as a patch graft and found graft patency for five month.

Research Products

• [Journal Article] Arterial graft with elastic layer structure grown from cells (2017)
  • Author(s): U. Yokoyama, Y. Tonooka, R. Koretake, T. Akimoto, Y. Gonda, J. Saito, M. Umemura, T. Fujita, S. Sakuma, F. Arai, M. Kaneko, and Y. Ishikawa
  • Journal Title: Scientific Reports Volume : 7 Pages : 140-140
  • DOI: 10.1038/s41598-017-00237-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enhanced EP4 Expression in a Pulmonary Artery Aneurysm With Dissection in a Patient With Pulmonary Arterial Hypertension (2017)
  • Author(s): S. Akagi, K. Nakamura, U. Yokoyama, S. Kasahara, T. Sarashina, K. Ejiri, H. Ito
  • Journal Title: Circulation: Cardiovascular Imaging Volume : 10
  • DOI: 10.1161/circimaging.116.005839
  • Peer Reviewed / Open Access
• [Journal Article] Pathology and molecular mechanisms of coarctation of the aorta and its association with the ductus arteriosus (2017)
  • Author(s): U. Yokoyama, Y. Ichikawa, S. Minamisawa, and Y. Ishikawa
  • Journal Title: The Journal of Physiological Sciences Volume : 67 Pages : 259-270
  • DOI: 10.1007/s12576-016-0512-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Glutamate promotes contraction of the rat ductus arteriosus. (2016)
  • Author(s): Fujita S, Yokoyama U, Ishiwata R, Aoki R, Nagao K, Masukawa D, Umemura M, Fujita T, Iwasaki S, Nishimaki S, Seki K, Ito S, Goshima Y, Asou T, Masuda M, Ishikawa Y. Glutamate promotes contraction of the rat ductus arteriosus.
  • Journal Title: Circ J Volume : 80 Pages : 2388-2396
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Use of Three-dimensional Arterial Models To Predict the In Vivo Behavior of Nanoparticles for Drug Delivery (2016)
  • Author(s): P. Chetprayoon, M. Matsusaki, U. Yokoyama, T. Tejima, Y. Ishikawa, M. Akashi
  • Journal Title: Angew. Chem. Int. Ed. Volume : 55 Pages : 4461-4466
  • DOI: 10.1002/anie.201509752
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Epac1 deficiency attenuated vascular smooth muscle cell migration and neointimal formation. (2015)
  • Author(s): Kato Y, Yokoyama U, Yanai C, Ishige R, Kurotaki D, Umemura M, Fujita T, Kubota T, Okumura S, Sata M, Tamura T, Ishikawa Y.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume : 35(12) Pages : 2617-2625
  • DOI: 10.1161/atvbaha.115.306534
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prostaglandin E-mediated molecular mechanisms driving remodeling of the ductus arteriosus. (2015)
  • Author(s): Yokoyama U.
  • Journal Title: Pediatr Int. Volume : 57(5) Pages : 820-827
  • DOI: 10.1111/ped.12769
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Norepinephrine-induced adrenergic activation strikingly increased the atrial fibrillation duration through β1- and α1-adrenergic receptor-mediated signaling in mice. (2015)
  • Author(s): Suita K, Fujita T, Hasegawa N, Cai W, Jin H, Hidaka Y, Prajapati R, Umemura M, Yokoyama U, Sato M, Okumura S, Ishikawa Y.
  • Journal Title: PLoS One. Volume : 23;10(7)
  • DOI: 10.1371/journal.pone.0133664
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Coupling of beta1-adrenergic receptor to type 5 adenylyl cyclase and its physiological relevance in cardiac myocytes. (2015)
  • Author(s): Tsunematsu T, Okumura S, Mototani Y, Ohnuki Y, Jin H, Cai W, SuitaK, Sato I, Umemura M, Yokoyama U, Sato M, Fujita T, and Ishikawa Y.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume : 458 Pages : 531-535
  • DOI: 10.1016/j.bbrc.2015.01.149
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oscillation of cAMP and Ca(2+) in cardiac myocytes: a systems biology approach (2015)
  • Author(s): Kamide T, Okumura S, Ghosh S, Shinoda Y, Mototani Y, Ohnuki Y, Jin H, Cai W, Suita K, Sato I, Umemura M, Fujita T, Yokoyama U, Sato M, Furutani K, Kitano H, Ishikawa Y
  • Journal Title: The Journal of Physiological Sciences Volume : 65(2) Pages : 195-200
  • DOI: 10.1007/s12576-014-0354-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Coupling of beta1-adrenergic receptor to type 5 adenylyl cyclase and its physiological relevance in cardiac myocytes. (2015)
  • Author(s): TsunematsuT, Okumura S, Mototani Y, Ohnuki Y, Jin H, Cai W, Suita K, Sato I, Umemura M, Yokoyama U, Sato M, Fujita T, Ishikawa Y.
  • Journal Title: Biochemical and Biophysical Research Communications (BBRC) Volume : -
  • Peer Reviewed / Open Access
• [Journal Article] Epac1-dependent phospholamban phosphorylation mediates the cardiac response to stresses. (2015)
  • Author(s): Okumura S, Fujita T, Cai W, Jin M, Namekata I, Mototani Y, Jin H, Ohnuki Y, Tsuneoka Y, Kurotani R, Suita K, Kawakami Y, Hamaguchi S, Abe T, Kiyonari H, Tsunematsu T, Bai Y, Suzuki S, Hidaka Y, Umemura M, Ichikawa Y, Yokoyama U, Sato M, Ishikawa F, Izumi-Nakaseko H, Adachi-Akahane S, Tanaka H, Ishikawa Y
  • Journal Title: Clin Invest. Volume : 124 Pages : 2785-2801
  • DOI: 10.1172/jci64784
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microfluidic perfusion culture system for multilayer artery tissue models (2014)
  • Author(s): Yuka Yamagishi, Taisuke Masuda, Michiya Matsusaki, Mitsuru Akashi, Utako Yokoyama, Fumihito Arai
  • Journal Title: Biomicrofluids Volume : 8 Pages : 064113-064113
  • DOI: 10.1063/1.4903210
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Decreased serum osmolality promotes ductus arteriosus constriction. (2014)
  • Author(s): Aoki R, Yokoyama U, Ichikawa Y, Taguri M, Kumagaya S, Ishiwata R, Yanai C, Fujita S, Umemura M, Fujita T, Okumura S, Sato M, Minamisawa S, Asou T, Masuda M, Iwasaki S, Nishimaki S, Seki K, Yokota S, Ishikawa Y.
  • Journal Title: Cardiovascuar Reaseach Volume : 104(2) Pages : 326-336
  • DOI: 10.1093/cvr/cvu199
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Thromboxane A(2) receptor stimulation promotes closure of the rat ductus arteriosus through enhancing neointima formation. (2014)
  • Author(s): Yokota T, Shiraishi R, Aida T, Iwai K, Liu NM, Yokoyama U, Minamisawa S.
  • Journal Title: PLoS One. Volume : VOL.9 Pages : 129-36
  • DOI: 10.1371/journal.pone.0094895
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protection of cardiomyocytes from the hypoxia-mediated injury by a Peptide targeting the activator of g-protein signaling 8 (2014)
  • Author(s): Sato M, Hiraoka M, Suzuki H, Sakima M, Mamun, AA, Yamane Y, Fujita T, Yokoyama U, Okumura S, and Ishikawa Y.
  • Journal Title: PLoS One Volume : 9
  • DOI: 10.1371/journal.pone.0091980
  • Peer Reviewed / Open Access
• [Journal Article] Combination therapy of cisplatin and inductive hyperthermia using ferucarbotran (Resovist®) in human oral cancer cells. (2014)
  • Author(s): Sato I, Umemura M, Mitsudo K, Kioi M, Nakashima H, Iwai T Fujita T, Yokoyama U, Okumura S, Feng X, Ito K, Miyajima A, Makino A, Iwai M, Eguchi H, I wai T, and Ishikawa Y.
  • Journal Title: The Journal of Physiological Sciences Volume : 64 Pages : 177-183
  • DOI: 10.1007/s12576-014-0309-8
  • Peer Reviewed / Open Access
• [Journal Article] Store-Operated Ca(2) (+) Entry (SOCE) Regulates Melanoma Proliferation and Cell Migration. (2014)
  • Author(s): Umemura M, Baljinnyam E, Feske S, De Lorenzo MS, Lai-Hua Xie LH, Feng X, 1, Itoh K, Makino A, Fujita T, Yokoyama U, Iw atsubo M, Chen S, Goydos JS, I shikawa Y, and Iwatsubo K
  • Journal Title: PLoS One. Volume : 9
  • DOI: 10.1371/journal.pone.0089292
  • Peer Reviewed / Open Access
• [Journal Article] Geranylgeranylacetone protects the heart via caveolae and caveolin-3 (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Hirokawa YT, Sakai Y, Hamaguchi E, Ishikawa Y, Yokoyama U, Kasai A, Kambe N, and Tanaka K.
  • Journal Title: Life Sci, Volume : 101 Pages : 43-48
  • DOI: 10.1016/j.lfs.2014.02.019
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional multilayers of smooth muscle cells as a new experimental model for vascular elastic fiber formation studies atherosclerosis. (2014)
  • Author(s): Ishiwata R, Yokoyama U, Matsusaki M, Yoshiya A, Kadowaki K, Ichikaww Y, Umemura M, Fujita T, Minamisawa S, Shimoda H, Mitsuru Akashi M, and Ishikawa Y.
  • Journal Title: Atherosclerosis, Volume : 233 Pages : 590-600
  • DOI: 10.1016/j.atherosclerosis.2014.01.045
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prostaglandin E2 Inhibits Elastogenesis in the Ductus Arteriosus via EP4 Signaling. (2014)
  • Author(s): Yokoyama U, Minamisawa S, Sh ioda A, Ishiwata R, Jin MH, Masuda M, Asou T, Sugimoto Y, Aoki II, Nakamura T, and Ishikawa Y
  • Journal Title: Circulation, Volume : 129 Pages : 487-486
  • DOI: 10.1161/circulationaha.113.004726
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] In vitro fabrication of functional arterial graft by supraphysiological hydrostatic pressurization. (2017)
  • Author(s): Yokoyama U, Saito J, Sakuma S, Arai F, Kaneko M, Ishikawa Y.
  • Organizer: The 81th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 石川音楽堂（石川県金沢市）
  • Year and Date: 2017-03-17
• [Presentation] ON-CHIP CELL GYM. (2017)
  • Author(s): Horade M, Kaneko M, Tsai CHD, Ito H, Higashino N, Akai T, Yokoyama U, Ishikawa Y, Sakuma S, Arai F.
  • Organizer: The 30th IEEE International Conference on Micro Electro Mechanical Systems
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2017-01-22
  • Int'l Joint Research
• [Presentation] Cell Exerciseによる細胞組織構築法はなぜいいのか (2016)
  • Author(s): 横山詩子
  • Organizer: 第２８回阪大医療組織工学フォーラム
  • Place of Presentation: 大阪大学（大阪府吹田市）
  • Invited
• [Presentation] 細胞工学による動脈グラフトの開発 (2016)
  • Author(s): 横山 詩子, 石川 義弘
  • Organizer: 第19回小児心血管分子医学研究会
  • Place of Presentation: 東京ドームホテル（東京都）
  • Invited
• [Presentation] Functional arterial graft fabricated from pressurized cell-layers. (2016)
  • Author(s): Ishikawa Y, Yokoyama U,Gonda Y, Akimoto T, Sakuma S, Arai F, Kaneko M.
  • Organizer: European Society of Cardiology
  • Place of Presentation: Rome, Italy
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] In-vitro fabrication of scaffold-free arterial grafts by periodic hydrodynamic pressurization. (2016)
  • Author(s): Yokoyama U, Tonooka Y, Koretake R, Akimoto T, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: International and Interdisciplinary Symposium 2016
  • Place of Presentation: 東京医科歯科大学（東京都）
  • Year and Date: 2016-07-11
  • Int'l Joint Research / Invited
• [Presentation] The behavior of cells during Cell Exercise. (2016)
  • Author(s): Higashino N, Kaneko M, Yokoyama U, Ishikawa Y
  • Organizer: ROBOMECH2016
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-08
• [Presentation] The behavior of different cell groups after the partition wall removal. (2016)
  • Author(s): Taniguchi T, Kaneko M, Yokoyama U.
  • Organizer: ROBOMECH2016
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-08
• [Presentation] The role of Epac1 in the regulatory mechanisms of vascular smooth muscle cell migration and neointimal formation. (2016)
  • Author(s): Kato Y, Yokoyama U, Ishikawa Y.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22
• [Presentation] Periodic hydrodynamic pressurization promotes layered structure of human arterial smooth muscle cells. (2016)
  • Author(s): Tonooka Y, Yokoyama U, Koretake R, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22
• [Presentation] Fabrication of layered elastic fibers in rat arterial constructs by periodic hydrodynamic pressurization. (2016)
  • Author(s): Gonda Y, Yokoyama U, Koretake R, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22
• [Presentation] 血管平滑筋と細胞外基質制御における新たなホスホリパーゼCとサイクリックAMPの役割The roles of phospholipase C and cyclic AMP in regulation of extracellular matrix in vascular smooth muscle cells. (2016)
  • Author(s): Yokoyama U.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22
  • Invited
• [Presentation] Inhibition of PGE2-EP4 Signaling as a Potential Future Therapeutic Strategy for Abdominal Aortic Aneurysm. (2016)
  • Author(s): Yokoyama U, Ishiwata R, Ichikawa Y, Yasuda S, Goda M, Suzuki S, Masuda M, Ishikawa Y.
  • Organizer: The 80th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 仙台国際センター（宮城県仙台市青葉区）
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] 早産児の動脈管はなぜ閉じないのか (2015)
  • Author(s): 横山詩子
  • Organizer: 第２８回北海道新生児談話会
  • Place of Presentation: 北海道大学病院（北海道札幌市北区）
  • Year and Date: 2015-11-15
  • Invited
• [Presentation] 動脈管閉鎖の分子機序と新たな薬物療法の可能性 (2015)
  • Author(s): 横山詩子
  • Organizer: 第２４回東海新生児研究会
  • Place of Presentation: 名古屋市立大学病院（愛知県名古屋市瑞穂区）
  • Year and Date: 2015-11-14
  • Invited
• [Presentation] プロスタグランディンE受容体―EP4を標的とした大動脈瘤治療の開発 (2015)
  • Author(s): 横山詩子
  • Organizer: 岡山心臓血管研究会
  • Place of Presentation: 岡山大学病院（岡山県岡山市北区）
  • Year and Date: 2015-09-30
  • Invited
• [Presentation] 平滑筋におけるPGE2-EP4シグナルは腹部大動脈瘤の慢性炎症をつかさどる (2015)
  • Author(s): 横山詩子、石渡遼、石川義弘
  • Organizer: 第２４６回生理学東京談話会
  • Place of Presentation: 東邦大学医学部（東京都大田区）
  • Year and Date: 2015-09-26
• [Presentation] 未熟児動脈管開存症治療におけるアミノ酸輸液組成の重要性 The effect of amino acids on patent ductus arteriosus in preterm infants (2015)
  • Author(s): 横山詩子、藤田秀次郎、青木理加、岩崎志穂、麻生俊英、益田宗孝、関和男、石川義弘
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2015-07-16
• [Presentation] ヒト動脈管における内膜肥厚部の遺伝子プロファイリングGene profiling of the intimal thickness in the human ductus arteriosus (2015)
  • Author(s): 齋藤純一、横山詩子、市川泰広、益田宗孝、麻生俊英、石川義弘
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2015-07-16
• [Presentation] 動脈管分化の分子メカニズム (2015)
  • Author(s): 横山詩子
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2015-07-16
  • Invited
• [Presentation] 平滑筋におけるPGE2-EP4シグナルは腹部大動脈瘤の発症を促進する (2015)
  • Author(s): 石渡遼、横山詩子、市川泰弘、石川義弘
  • Organizer: 第132回日本薬理学会関東部会
  • Place of Presentation: 明海大学浦安キャンパス（千葉県浦安市）
  • Year and Date: 2015-07-04
• [Presentation] 動脈管開存症治療における輸液組成の重要性 (2015)
  • Author(s): 横山詩子
  • Organizer: 神奈川県立こども医療センター第２４回心臓血管外科勉強会
  • Place of Presentation: 神奈川県立こども医療センター（神奈川県横浜市南区）
  • Year and Date: 2015-06-12
  • Invited
• [Presentation] Regulation of collagens in the heart. (2015)
  • Author(s): Yokoyama U.
  • Organizer: The 79th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市北区）
  • Year and Date: 2015-04-25
  • Invited
• [Presentation] Plasminogen activator may promote intimal thickening in the ductus arteriosus. (2015)
  • Author(s): Nicho N, Yokoyama U, Saitoh J, Yanai C, Ichikawa Y, Masuda M, Aso T, Ishikawa Y.
  • Organizer: The 118th Annual Meeting of the Japan Pediatric Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市北区）
  • Year and Date: 2015-04-17
• [Presentation] 実験的アプローチによる新たな動脈管開存症治療の開発 (2015)
  • Author(s): 横山詩子
  • Organizer: 第一回新生児科指導医養成事業による教育セミナー
  • Place of Presentation: 東北大学病院（宮城県仙台市）
  • Year and Date: 2015-03-28
  • Invited
• [Presentation] Fabrication of tissue-engineered human arterial constructs by cyclic hydrostatic pressure. (2015)
  • Author(s): Tonooka Y, Yokoyama U, Koretake R, Ishiwata R, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: The 92th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] ナノ薄膜技術を用いた高弾性を有する動脈グラフトの作製 Construction of biological elastic vessels by extracellular matrix nanofilm-based cell accumulation technique. (2015)
  • Author(s): Yokoyama U.
  • Organizer: he 92th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] PGE2-EP4 sigaling promotes vascular intimal thickening via fibulin-1. (2015)
  • Author(s): Yokoyama U, Yanai C, Sato A, Ishikawa Y.
  • Organizer: The 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] Secretome-based identification of PGE2-regulated proteins in human abdominal aortic aneurysm. (2015)
  • Author(s): Ishiwata R, Yokoyama U, Arakawa N, Ishikawa Y.
  • Organizer: The 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] PGE2-EP4 exerts an anti-fibrotic effect in the heart. (2015)
  • Author(s): Ishiwata R. Yokoyama U, Inoue S, Ichikawa Y, Ishikawa Y.
  • Organizer: he 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] PGE2-EP4 sigaling in smooth muscle cell plays a role in chronic inflammation of aortic abdominal aneurysm. (2015)
  • Author(s): Yokoyama U, Ichikawa Y, Ishiwata R, Ishikawa Y.
  • Organizer: The 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
  • Invited
• [Presentation] プロスタグランディンE－EP4はfibulin-1を介して生理的・病的血管内膜肥厚の形成に関与する Prostaglandin E2-EP4 is involved in physiological and pathological intimal thickening via fibulin-1 (2014)
  • Author(s): 矢内千春、横山詩子、熊谷駿、手島隆之、佐藤綾美、石川義弘
  • Organizer: 第13回日本心臓血管発生研究会
  • Place of Presentation: 磐梯熱海温泉 ホテル 華の湯（福島県郡山市）
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] Prostaglandin E2-EP4 plays a protective role against cardiac fibrosis. (2014)
  • Author(s): Ishiwata R, Yokoyama U, Inoue S, Ichikawa Y, Ishikawa Y.
  • Organizer: European Society of Cardiology
  • Place of Presentation: Barcerona, Spain
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] 動脈管は面白い (2014)
  • Author(s): 横山詩子
  • Organizer: 第24回心研小児科夏季セミナー
  • Place of Presentation: 東京女子医科大学（東京都新宿区）
  • Year and Date: 2014-07-26 – 2014-07-27
  • Invited
• [Presentation] 仕事の多様化について (2014)
  • Author(s): 横山詩子
  • Organizer: 横浜市立大学第２回Joinの会
  • Place of Presentation: 横浜市立大学附属市民総合医療センター(神奈川県横浜市)
  • Year and Date: 2014-07-19
  • Invited
• [Presentation] プロスタグランディンEによる血管弾性線維の制御メカニズム (2014)
  • Author(s): 横山詩子
  • Organizer: 東京農工大学 材料健康科学「Cell to Body Dynamicsセミナー」
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2014-07-18
  • Invited
• [Presentation] グルタミン酸が動脈管閉鎖に及ぼす作用の検討 (2014)
  • Author(s): 藤田秀次郎、横山詩子、青木理加、岩崎志穂、西巻滋、関和男、横田俊平、石川義弘
  • Organizer: 第50回日本周産期・新生児医学会学術集会
  • Place of Presentation: シェラトングランデトーキョーベイ（千葉県浦安市）
  • Year and Date: 2014-07-13 – 2014-07-15
• [Presentation] Early Aggressive Parenteral Nutritionの問題点・PDA (2014)
  • Author(s): 横山詩子
  • Organizer: 第50回日本周産期・新生児医学会学術集会
  • Place of Presentation: シェラトングランデトーキョーベイ（千葉県浦安市）
  • Year and Date: 2014-07-13 – 2014-07-15
  • Invited
• [Presentation] ヒト大動脈瘤組織におけるPGE2-EP4誘導性分泌タンパク質の網羅的解析 (2014)
  • Author(s): 石渡遼、横山詩子、荒川憲昭、安田章沢、郷田素彦、鈴木伸一、益田宗孝、石川義弘
  • Organizer: 第35回日本循環制御医学会総会
  • Place of Presentation: 福岡大学医学部百年講堂（福岡県福岡市）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] Nuclear Factor kappa B Inhibition Promotes Closure of the Rat Ductus Arteriosus. (2014)
  • Author(s): Kajimura I, Yokoyama U, Ishikawa Y, Minamisawa S.
  • Organizer: Experimental Biology Annual Meetin
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] cAMP reverses the pro-fibrogenic phenotype of cardiac myofibroblasts. (2014)
  • Author(s): Aroonsakool N, Yokoyama U, Lu D, Patel HH, Insel PA.
  • Organizer: Experimental Biology Annual Meetin
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] Prostaglandin E2 receptor EP4 signaling in vascular smooth muscle cells decreases aortic elasticity. (2014)
  • Author(s): Ichikawa Y, Yokoyama U, Ishikawa Y.
  • Organizer: Experimental Biology Annual Meetin
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Book] Lipid Mediators. Current Reviews and Protocols. (2015)
  • Author(s): Yokoyama U, Ishiwata R, Ishikawa Y.
  • Total Pages: 11
  • Publisher: Springer
• [Book] Etiology and Morphogenesis of Congenital Heart Disease. (2015)
  • Author(s): Yokoyama U*, Minamisawa S, Ishikawa Y.
  • Publisher: Springer
• [Book] Nanomedicine and Nanotoxicology. (2014)
  • Author(s): Yokoyama U*, Ishikawa Y.
  • Total Pages: 271
  • Publisher: Springer
• [Remarks] 横浜市立大学医学部循環制御医学
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/
• [Remarks] 横浜市立大学医学部循環制御医学
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/index.html
• [Patent(Industrial Property Rights)] 三次元組織体及びその製造方法 (2016)
  • Inventor(s): 明石満、松崎典弥、石川義弘、横山詩子
  • Industrial Property Rights Holder: 明石満、松崎典弥、石川義弘、横山詩子
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-04-08
  • Application Year: 2016
• [Patent(Industrial Property Rights)] 三次元組織体及びその製造方法 (2016)
  • Inventor(s): 明石満、松崎典弥、石川義弘、横山詩子
  • Industrial Property Rights Holder: 明石満、松崎典弥、石川義弘、横山詩子
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-04-10
  • Application Year: 2016
  • Overseas
• [Patent(Industrial Property Rights)] 三次元細胞集合体の作製方法 (2015)
  • Inventor(s): 横山詩子、石川義弘、金子真、佐久間臣耶、新井史人
  • Industrial Property Rights Holder: 横山詩子、石川義弘、金子真、佐久間臣耶、新井史人
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-09-29
  • Application Year: 2015
  • Overseas