| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
In order to generate human insulin-resistant cells, we try to establish human iPS cells derived from a patient with insulin receptor gene mutations (Leprechaunism) and to differentiate them into insulin responsive cells, including hepatocytes or adipocytes. After establishment of them, we investigate the effects of low molecular weight chemicals that inhibits Foxo1 transcriptional activity using these cells because Foxo1 activity in these cells are active due to defects of insulin signaling.
We could establish human iPS cells from a patient with Leprechaunism but not make them differentiate into insulin-responsive hepatocyte and adipocytes. However, I could have nine low molecular weight chemicals that activate or inhibit Foxo1 transcriptional activity. Therefore, we will continue to identify chemicals for therapy of type 2 diabetes mellitus.
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