Analysis of a protein localized at stratified epithelial cell junction

• Project/Area Number: 26670527
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Nagoya University
• Principal Investigator: Hirako Yoshiaki 名古屋大学, 理学研究科, 講師 (50377909)
• Co-Investigator(Renkei-kenkyūsha): NISHIZAWA Yuji 中部大学, 生命健康科学部, 教授 (80252229) ; AOYAMA Yumi 川崎医科大学, 医学部, 教授 (90291393)
• Research Collaborator: IWATSUKA Tomohiro ; MATSUDA Kazuhisa
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞間接着 / 表皮分化 / 細胞接着 / ケラチノサイト / 表皮

Outline of Final Research Achievements

Hemidesmosomes and desmosomes in epidermis contain proteins that are not found in their counterparts in simple epithelia. In this study, we have analyzed a novel cell-cell junction protein that shows unique tissue distribution. Fist, we produced a monoclonal antibody specific to the protein. Using this antibody we demonstrated its differentiation-dependent expression in stratified epithelia. We further analyzed the mechanism that target the protein to cell-cell adhesion site by transient transfections of cDNA constructs encoding various mutants of the protein.

Research Products

• [Journal Article] Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid. (2016)
  • Author(s): Li X, Qian H, Sogame R, Hirako Y, Tsuruta D, Ishii N, Koga H, Tsuchisaka A, Jin Z, Tsubota K, Fukumoto A, Sotozono C, Kinoshita S, Hashimoto T
  • Journal Title: Eur J Dermatol Volume: 26(3) Issue: 3 Pages: 247-253
  • DOI: 10.1684/ejd.2016.2772
  • Peer Reviewed
• [Journal Article] β4 integrin in hereditary and acquired mucocutaneous diseases. (2016)
  • Author(s): Hashimoto T, Hirako Y, Tsuruta D.
  • Journal Title: Exp Dermatol. Volume: 25 Issue: 4 Pages: 267-268
  • DOI: 10.1111/exd.12961
• [Journal Article] A case of subepidermal blistering disease with autoantibodies to multiple laminin subunits who developed later autoantibodies to alpha-5 chain of type IV collagen associated with membranous glomerulonephropathy. (2015)
  • Author(s): Sueki H, Sato Y, Ohtoshi S, Nakada T, Yoshimura A, Tateishi C, Borza DB, Fader W, Ghohestani RF, Hirako Y, Koga H, Ishii N, Tsuchisaka A, Qian H, Li X, Hashimoto T
  • Journal Title: Acta Derm Venereol Volume: 95 Pages: 826-829
  • DOI: 10.2340/00015555-2059
  • Peer Reviewed
• [Journal Article] Linear IgA/IgG bullous dermatosis reacts with multiple laminins and integrins. (2015)
  • Author(s): Li X, Tsuchisaka A, Qian H, Teye K, Ishii N, Sogame R, Harada K, Nakagomi D, Shimada S, Tateishi C, Hirako Y, Hashimoto T
  • Journal Title: Eur J Dermatol Volume: 25 Issue: 5 Pages: 418-423
  • DOI: 10.1684/ejd.2015.2555
  • Peer Reviewed
• [Journal Article] Pure ocular mucous membrane pemphigoid reactive with both β4 integrin and the BP180 C-terminal domain. (2015)
  • Author(s): Solano-Lopez G, Concha-Garzon MJ, Sanchez-Perez J, Hirako Y, Li X, Ishii N, Hashimoto T, Dauden E.
  • Journal Title: Br J Dermatol Volume: 172 Issue: 2 Pages: 542-544
  • DOI: 10.1111/bjd.13290
  • Peer Reviewed
• [Journal Article] N-linked glycosylation on laminin γ1 influences recognition of anti-laminin γ1 pemphigoid autoantibodies. (2015)
  • Author(s): Li X, Qian H, Takizawa M, Koga H, Tsuchisaka A, Ishii N, Hayakawa T, Ohara K, Sitaru C, Zillikens D, Sekiguchi K, Hirako Y, Hashimoto T.
  • Journal Title: J Dermatol Sci Volume: 77 Issue: 2 Pages: 125-129
  • DOI: 10.1016/j.jdermsci.2014.12.003
  • Peer Reviewed
• [Journal Article] Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line (2014)
  • Author(s): Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, and Owaribe K
  • Journal Title: Exp Cell Res Volume: 324(2) Issue: 2 Pages: 172-182
  • DOI: 10.1016/j.yexcr.2014.04.002
  • Peer Reviewed
• [Journal Article] A case of concurrent anti-laminin γ1 pemphigoid and anti-laminin 332-type mucous membrane pemphigoid (2014)
  • Author(s): Li X, Qian H, Ishii N, Yamaya M, Fukuda H, Mukai H, Hirako Y, and Hashimoto T
  • Journal Title: Br J Dermatol Volume: 171 Issue: 6 Pages: 1555-1557
  • DOI: 10.1111/bjd.13113
  • Peer Reviewed
• [Journal Article] Major cleavage-dependent epitopes for linear IgA bullous dermatosis are formed at the boundary between the non-collagenous 16A and collagenous 15 domains of BP180. (2014)
  • Author(s): Yamauchi T, Matsushita S, Hashimoto T, Hirako Y.
  • Journal Title: J Dermatol Sci Volume: 76 Issue: 1 Pages: 25-33
  • DOI: 10.1016/j.jdermsci.2014.07.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A case of concurrent antilaminin γ1 pemphigoid and antilaminin-332-type mucous membrane pemphigoid. (2014)
  • Author(s): Li X, Qian H, Ishii N, Yamaya M, Fukuda H, Mukai H, Hirako Y, Hashimoto T.
  • Journal Title: Br J Dermatol Volume: 171 Issue: 5 Pages: 1257-1259
  • DOI: 10.1111/bjd.13107
  • Peer Reviewed
• [Presentation] Knockout of type XVII collagen in DJM-1 cells affects structure of type I hemidesmosomes and deposition pattern of laminin-332 matrix (2016)
  • Author(s): 山内友恵、加茂遼太郎、平子善章
  • Organizer: The 27th CDB meeting "Body Surface Tactics"
  • Place of Presentation: 理研CDB神戸
  • Year and Date: 2016-11-14
• [Presentation] XVII型コラーゲンを欠失したDJM-1細胞は、I型ヘミデスモソーム形成とラミニン332マトリックス沈着パターンに異常を示す (2016)
  • Author(s): 山内友恵、加茂遼太郎、平子善章
  • Organizer: 第68回日本細胞生物学会大会
  • Place of Presentation: 京都メルサ
  • Year and Date: 2016-06-15
• [Presentation] DJM-1 cells deficient in BP180/type XVII collagen exhibit rudiment hemidesmosomes and abnormal pattern of laminin-332 matrix (2015)
  • Author(s): 平子善章、加茂遼太郎、金田みのり
  • Organizer: 第４０回日本研究皮膚科学会
  • Place of Presentation: 岡山市岡山コンベンションセンター
  • Year and Date: 2015-12-11
• [Presentation] DJM-1細胞を用いたヘミデスモソームの基礎研究 (2015)
  • Author(s): 平子善章
  • Organizer: 第３７回水疱症研究会
  • Place of Presentation: 福島市コラッセ福島
  • Year and Date: 2015-09-26
  • Invited
• [Presentation] XVII型コラーゲンの欠失はI型ヘミデスモソーム形成とラミニン３３２マトリクスの沈着パターンに影響する (2015)
  • Author(s): 平子善章、加茂遼太郎
  • Organizer: 第４７回日本結合組織学会学術大会 
  • Place of Presentation: 東京都港区コクヨホール
  • Year and Date: 2015-05-15
• [Presentation] Major cleavage-dependent epitopes for linear IgA bullous dermatosis are formed at the boundary between the NC16A and C15 domains of BP180 (2014)
  • Author(s): 平子善章、山内友恵、松下知嗣、橋本隆
  • Organizer: 第39回日本研究皮膚科学会学術大会
  • Place of Presentation: 大阪府大阪市 ホテル阪急エキスポパーク
  • Year and Date: 2014-12-12 – 2014-12-14
• [Presentation] A low calcium culture condition promotes deposition of laminin-332 and formation of hemidesmosomes in a squamous carcinoma cell line (2014)
  • Author(s): Yoshiaki Hirako, Yuki Yonemoto, Tomoe Yamauchi, Yuji Nishizawa, Yoshiyuki Kawamoto, Katsushi Owaribe
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県奈良市 奈良県新公会堂
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] プロテアーゼ消化によりXVII型コラーゲンの細胞外部分には自己抗原性エピトープが形成される (2014)
  • Author(s): 山内友恵、松下知嗣、橋本隆、平子善章
  • Organizer: 第46回日本結合組織学会・第61回マトリックス研究会 合同学術集会
  • Place of Presentation: 愛知県名古屋市ウィンクあいち
  • Year and Date: 2014-06-05 – 2014-06-07
• [Remarks] 名古屋大学大学院理学研究科生命理学専攻 論文紹介ページ
  • URL: http://www.bio.nagoya-u.ac.jp/paper/index.html