Biological markers of plasma protein glycosylation in major depressive disorder

• Project/Area Number: 26670542
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Yamaguchi University
• Principal Investigator: YAMAGATA Hirotaka 山口大学, 医学部附属病院, 講師 (10549934)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: うつ病 / バイオマーカー / 糖鎖

Outline of Final Research Achievements

Glycosylation is one of the most common posttranslational modifications that occur during protein biosynthesis, and protein glycosylation is a major contributor to many diseases. However, the mechanisms of protein glycosylation in the pathophysiology of major depressive disorder have not been elucidated. We analyzed plasma protein glycosylation using a lectin microarray, and also examined protein plasma glycosylation in a mouse model of depression. Our results describe lectins that are associated with depression and stress.

Research Products

• [Journal Article] Hippocampal Sirtuin 1 Signaling Mediates Depression-like Behavior (2016)
  • Author(s): Naoko Abe-Higuchi, Shusaku Uchida, Hirotaka Yamagata, Fumihiro Higuchi, Teruyuki Hobara, Kumiko Hara, Ayumi Kobayashi, and Yoshifumi Watanabe
  • Journal Title: Biological Psychiatry Volume: In press Issue: 11 Pages: 815-826
  • DOI: 10.1016/j.biopsych.2016.01.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Blood transcriptomic markers in patients with late-onset major depressive disorder (2016)
  • Author(s): Miyata S, Kurachi M, Okano Y, Sakurai N, Kobayashi A, Harada K, Yamagata H, Matsuo K, Takahashi K, Narita K, Fukuda M, Ishizaki Y, Mikuni M
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0150262-e0150262
  • DOI: 10.1371/journal.pone.0150262
  • Peer Reviewed / Open Access
• [Journal Article] Genetic Demonstration of a Role for Stathmin in Adult Hippocampal Neurogenesis, Spinogenesis, and NMDA Receptor-Dependent Memory (2016)
  • Author(s): Martel G, Uchida S, Hevi C, Chevere-Torres I, Fuentes I, Park YJ, Hafeez H, Yamagata H, Watanabe Y, Shumyatsky GP
  • Journal Title: J Neurosci Volume: 36 Issue: 4 Pages: 1185-1202
  • DOI: 10.1523/jneurosci.4541-14.2016
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Histone deacetylase mediates the decrease in drebrin cluster density induced by amyloid beta oligomers. (2014)
  • Author(s): Ishizuka Y, Shimizu H, Takagi E, Kato M, Yamagata H, Mikuni M, Shirao T
  • Journal Title: Neurochem Int. Volume: 76 Pages: 114-21
  • DOI: 10.1016/j.neunet.2014.07.005
  • Peer Reviewed
• [Journal Article] Imipramine ameliorates pain-related negative emotion via induction of brain-derived neurotrophic factor (2014)
  • Author(s): S Yasuda, M Yoshida, H Yamagata, Y Iwanaga, H Suenaga, K Ishikawa, M Nakano, S Okuyama, Y Furukawa, S Furukawa, T Ishikawa
  • Journal Title: Cell Mol Neurobiol Volume: 34 Issue: 8 Pages: 1199-1208
  • DOI: 10.1007/s10571-014-0097-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] The analysis of murine plasma proteins after chronic stress by lectin microarray (2015)
  • Author(s): Hirotaka Yamagata, Shusaku Uchida, Fumihiro Higuchi, Teruyuki Hobara, Yoshifumi Watanabe
  • Organizer: Neuroscience2015
  • Place of Presentation: McCormick Place, Chicago, USA
  • Year and Date: 2015-10-19
  • Int'l Joint Research
• [Presentation] Distinction of brain morphometric abnormalites in bipolar and unipolar depression: A voxel-based multi-center sturdy (2015)
  • Author(s): Koji Matsuo, Kenichiro Harada, Yasumasa Okamoto, Miho Ohta, Hisashi Narita, Yusuke Fujita, Go Okada, Masahiro Takamura, Hirotaka Yamagata, Ichiro Kusumi, Takeshi Inoue, Hiroshi Kunugi, Shigeto Yamawaki, Yoshifumi Watanabe
  • Organizer: Neuroscience2015
  • Place of Presentation: McCormick Place, Chicago, USA
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] 高齢者うつ病患者における内側前頭‐辺縁系の構造異常 (2015)
  • Author(s): 原田健一郎、松尾幸治、中島麻美、樋口文宏、樋口尚子、柴田朋彦、芳原輝之、中野雅之、大朏孝治、綿貫俊夫、松原敏郎、古川又一、藤田悠介、下地啓五、松永尚文、山形弘隆、渡邉義文
  • Organizer: 第37回日本生物学的精神医学会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-09-25
• [Presentation] エピジェネティクスによるうつ病の分子･細胞基盤 (2015)
  • Author(s): 内田周作, 芳原輝之, 樋口文宏, 樋口尚子, 山形弘隆, 渡邉義文
  • Organizer: 第37回日本生物学的精神医学会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-09-25
• [Presentation] レクチンアレイを用いたうつ病患者血漿タン パク質の糖鎖解析 (2015)
  • Author(s): 山形弘隆、内田周作、芳原輝之、樋口文宏、原田健一郎、松尾幸治、三國雅彦、渡邉義文
  • Organizer: 第37回日本生物学的精神医学会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-09-25
• [Presentation] レジリエンスの視点からうつ病の発症・回復の分子メカニズムを理解する (2015)
  • Author(s): 内田周作, 樋口尚子, 山形弘隆, 芳原輝之, 樋口文宏, 渡邉義文
  • Organizer: 第37回日本生物学的精神医学会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-09-24
• [Presentation] Different fronto-temporal activation during an emotional word task in patients with unipolar and bipolar depression: a functional near-infrared spectroscopy study (2015)
  • Author(s): Toshio Matsubara, Koji Matsuo, Kenichiro Harada, Mami Nakashima, Masayuki Nakano, Masako Hirotsu, Naoko Higuchi, Toshio Watanuki, Fumihiro Higuchi, Koji Otsuki, Tomohiko Shibata, Teruyuki Hobara, Hirotaka Yamagata, Yoshifumi Watanabe
  • Organizer: Society of Biological Psychiatry 70th Annual Meeting
  • Place of Presentation: Fairmont Royal York, Toronto, Canada
  • Year and Date: 2015-05-16
  • Int'l Joint Research
• [Presentation] Reduced gray-matter volume of fronto-temporal cortex in patients with late-life depression (2015)
  • Author(s): Kenichiro Harada, Koji Matsuo, Hirotaka Yamagata, Mami Nakashima, Masayuki Nakano, Naoko Higuchi, Fumihiro Higuchi, Teruyuki Hobara, Koji Otsuki, Tomohiko Shibata, Toshio Watanuki, Katsuhiko Ueda, Matakazu Furukawa, Masaki Fukunaga, Yusuke Fujita, Keigo Shimoji, Toshio Matstbara, Naofumi Matsunaga, Yoshifumi Watanabe
  • Organizer: Society of Biological Psychiatry 70th Annual Meeting
  • Place of Presentation: Fairmont Royal York, Toronto, Canada
  • Year and Date: 2015-05-16
  • Int'l Joint Research
• [Presentation] Gene expression change in the hippocampus of BALB/c mice after chronic ultra-mild stress (2014)
  • Author(s): Yamagata H, Hobara T, Uchida S, Higuchi F, Higuchi N, Watanabe Y
  • Organizer: Neuroscience 2014, SfN's 44th annual meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2014-11-19
• [Presentation] Essential role of hippocampal SIRT1 in behavioral response to stress in mice (2014)
  • Author(s): Uchida S, Higuchi N, Yamagata H, Hobara T, Higuchi F, Hara K, Kobayashi A, Watanabe Y
  • Organizer: Neuroscience 2014, SfN's 44th annual meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2014-11-19
• [Presentation] Upregulation of CaMKIIβ by HDAC inhibitors might be involved in structural plasticity and behavioral responses in model mouse of depression (2014)
  • Author(s): Hobara T, Uchida S, Higuchi F, Higuchi N, Yamagata H, Watanabe Y
  • Organizer: Neuroscience 2014, SfN's 44th annual meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2014-11-19
• [Presentation] Identical blood biomarkers in late-onset major depressive disorder patients and model mice (2014)
  • Author(s): Miyata S, Kurachi M, Sakurai N, Takashi K, Yamagata H, Matsuo K, Narita K, Fukuda M, Ishizaki Y, Mikuni M
  • Organizer: Neuroscience 2014, SfN's 44th annual meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2014-11-18
• [Presentation] Low dose of imipramine ameliorates pain-related negative emotion via induction of brain-derived neurotrophic factor (2014)
  • Author(s): Okuyama S, Yasuda S, Yamagata H, Fukuhara K, Inagawa Y, Nakano M, Ishikawa K, Furukawa Y, Watanabe Y, Ishikawa T
  • Organizer: Neuroscience 2014, SfN's 44th annual meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2014-11-16
• [Presentation] 慢性ストレス負荷BALB/cマウスの海馬における遺伝子発現マイクロアレイ解析 (2014)
  • Author(s): 山形 弘隆, 内田 周作, 芳原 輝之, 樋口 文宏, 渡邉 義文
  • Organizer: 第36回日本生物学的精神医学会
  • Place of Presentation: 奈良市文化会館（奈良市）
  • Year and Date: 2014-09-30
• [Presentation] 海馬未成熟神経細胞の形態変化によるうつ様行動の変化 (2014)
  • Author(s): 芳原 輝之, 松ケ谷 佳代, 内田 周作, 山形 弘隆, 樋口 文宏, 渡辺 義文
  • Organizer: 第36回日本生物学的精神医学会
  • Place of Presentation: 奈良市文化会館（奈良市）
  • Year and Date: 2014-09-30
• [Presentation] うつ病様行動と神経可塑性におけるmicroRNA-124とHDAC7の役割の解析 (2014)
  • Author(s): 樋口 文宏, 内田 周作, 山形 弘隆, 大朏 孝治, 芳原 輝之, 樋口 尚子, 柴田 朋彦, 渡邉 義文
  • Organizer: 第36回日本生物学的精神医学会
  • Place of Presentation: 奈良市文化会館（奈良市）
  • Year and Date: 2014-09-29