| Project/Area Number |
26670548
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Tsuboi Koji 筑波大学, 医学医療系, 教授 (90188615)
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| Co-Investigator(Kenkyū-buntansha) |
ZENKOH Junko 筑波大学, 医学医療系, 研究員 (20710148)
ITO Atsuo 国立研究開発法人産業技術総合研究所, 生命工学領域 健康工学研究部門 生体材料研究グループ, 研究グループ長 (30356480)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 放射線治療生物学 / 免疫応答細胞死 |
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| Outline of Final Research Achievements |
Localized radiotherapy for cancers has an effect of immune adjuvant by inducing immunogenic cell death. The signals from dying or newly dead cells evoke anti-tumor immunity that is mediated by activated CTLs.
In this study, murine glioma cells GL261 were inoculated to syngeneic albino C57BL/6 mice. Mesoporous silica nanoparticles (MSNs) were used as an immune adjuvant. Intra-tumoral injection of MSNs was combined with localized X-ray irradiation, and the tumor growth rates and survival were analyzed. In addition, the immune-enhancing effect of indomethacin was examined.
Consequently, MSNs administration (0.1 mg/mouse) once a week for 3 weeks delayed tumor growth significantly. Moreover, 6 times administrations induced complete cure in 90% of the treated mice inducing abscopal effect in the brain. Furthermore, sequential oral administration of indomethacin enhanced anti-tumor effect and prolonged survivals of the mice. MSNs may be a promising candidate for a novel immune adjuvant.
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