| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Among the several IR-induced mutations, chromosomal translocation is one of the critical mutations, however, the conventional assay requires a highly-trained skill. Here, we aim to establish a new method to measure chromosomal translocation by using a site specific endonuclease, I-Ppo1. HT1080 cells, which express an inducible I-Ppo1 vector, were used in this study. I-PpoI was induced by 4-OHT treatment. The efficiency of translocation was accessed by PCR using primer sets between rDNA and DAB1 locus, however, an obvious signal was not detected by the primer sets. By considering other data, we concluded that more efficient digestion is required to detect I-PpoI induced translocation.
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