Spatiotemporal analyses of the dynamics of hypoxic tumor cells for the optimization of fractionated radiation therapy for cancers
| Project/Area Number |
26670558
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Kyoto University |
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Principal Investigator |
Harada Hiroshi 京都大学, 白眉センター, 准教授 (80362531)
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| Co-Investigator(Kenkyū-buntansha) |
Yoshimura Michio 京都大学, 大学院医学研究科, 助教 (40597936)
KOBAYASHI Minoru 京都大学, 大学院医学研究科, 研究員 (40644894)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | がん / 放射線治療 / 分割照射 / 腫瘍内低酸素 / HIF-1 / 放射線治療抵抗性 / 低酸素 / 細胞動態 |
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| Outline of Final Research Achievements |
In the present study, we exploited a sophisticated strategy which enabled us to tag hypoxic cells with luciferase protein in tumor xenograft by using the hypoxia-responsive transcription factor-dependent Cre-loxP system, and performed spatiotemporal analyses about the dynamics of cancer cells in intratumoral hypoxic regions. What we found is that, 24 hours after hypoxic cells were tagged with luciferase protein in the regions 70-85 micrometers distant regions from tumor blood vessels, the cells were located in 85-100 micrometers from vessels. In the following day (48 hours after the tagging), the cells were detected in necrotic regions located more than 100 micrometer from vessels. These results support an idea that the interval of fractionated radiation therapy for cancer is about 24 hours.
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Report
(3 results)
Research Products
(35 results)
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[Journal Article] Evaluation of Tumor-associated Stroma and Its Relationship with Tumor Hypoxia Using Dynamic Contrast-enhanced CT and 18F Misonidazole PET in Murine Tumor Models.2016
Author(s)
Koyasu, S., Tsuji, Y., Harada, H., Nakamoto, Y., Nobashi, T., Kimura, H., Sano, K., Koizumi, K., Hamaji, M., Togashi, K.
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Journal Title
Radiology.
Volume: 278
Issue: 3
Pages: 734-41
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] UCHL1 provides diagnostic and antimetastatic strategies due to its deubiquitinating effect on HIF-1alpha2015
Author(s)
Goto Y, Zeng L, YeomCJ, Zhu Y, Morinibu A, Shinoyam K, Kobayashi M, Hirota K, Itasaka S, Yoshimura M, Tanimoto K, Sowa T, Menju T, Sonobe M, Kakeya H, Toi M, Date H, Hammond EM, Hiraoka M, Harada H.
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Journal Title
Nat Commun
Volume: 6
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Aberrant IDH3α expression promotes malignant tumor growth by inducing HIF-1-mediated metabolic reprogramming and angiogenesis.2014
Author(s)
Zeng L, Morinibu A, Kobayashi M, Zhu Y, Wang X, Goto Y, Yeom CJ, Zhao T,Hirota K, Shinomiya K, Itasaka S, Yoshimura M, Guo G, Hammond EM, Hiraoka M, Harada H.
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Journal Title
Oncogene
Volume: epub
Issue: 36
Pages: 4758-4766
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Near-infraredfluorescence imaging of cancer mediated by tumor hypoxia and HIF1α/OATPs signaling axis.2014
Author(s)
Wu JB, Shao C, Li X, Shi C, Li Q, Hu P, Chen YT, Dou X, Sahu D, Li W, Harada H, Zhang Y, Wang R, Zhau HE, Chung LW.
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Journal Title
Biomaterials
Volume: 35
Issue: 28
Pages: 8175-8185
DOI
Related Report
Peer Reviewed
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