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Identification of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening

Research Project

Project/Area Number 26670559
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionOsaka University

Principal Investigator

Tamari Keisuke  大阪大学, 医学(系)研究科(研究院), 特任助教(常勤) (30718995)

Co-Investigator(Kenkyū-buntansha) SEO Yuji  大阪大学, 医学系研究科, 特任助教 (00302000)
ISOHASHI Fumiaki  大阪大学, 医学系研究科, 特任准教授 (00448036)
OGAWA Kazuhiko  大阪大学, 医学系研究科, 教授 (40253984)
HAYASHI Kazuhiko  大阪大学, 医学系研究科, 招へい教員 (70718981)
Co-Investigator(Renkei-kenkyūsha) ISHII Hideshi  大阪大学, 医学系研究科, 特任教授 (10280736)
Kim Yoon-Jeong  大阪大学, 産学連携本部, 教授 (40574330)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords放射線増感剤 / 化合物スクリニーニング / 放射線治療 / 癌幹細胞 / 創薬 / 放射線増感
Outline of Final Research Achievements

Cancer recurrence and metastases can be due to a subpopulation of cells with stem-like characteristics, cancer stem cells (CSCs) which maintain the capacity to regenerate entire tumors. We suspected that CSC-specific compounds had radiosensitizing effects in parental cells. To accomplish this purpose, we performed high throughput screening (HTS) to identify compounds that specifically act CSCs. Finally, we found five CSC-specific compounds. However, these compounds had no significant radiosensitizing effects for parental HeLa cells. We also performed cell-based high throughput screening to study new radiosensitizers targeting hypoxic tumor cells. We identified 22 compounds as candidate. Clonogenic survival assay showed their significant radiosensitizing effect in hypoxic condition. In conclusion, we identified new radiosensitizers by high throughput screening. This is a possible method for identifying radiosensitizers targeting hypoxic cancer cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] A novel method to identify radiosensitizers targeting hypoxic cancer cells by high throughput screening2016

    • Author(s)
      Tamari K, Seo Y, Takahashi Y, Suzuki O, Isohashi F, Yoshioka Y, and Ogawa K.
    • Organizer
      AACR Annual Meeting 2016
    • Place of Presentation
      Ernest N. Morial Convention Center New Orleans, Louisiana
    • Year and Date
      2016-04-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of novel radiosensitizers targeting hypoxic cancer cells by high throughput screening2016

    • Author(s)
      Tamari K, Seo Y, Takahashi Y, Suzuki O, Isohashi F, Yoshioka Y, and Ogawa K.
    • Organizer
      Tenth AACR-JCA Joint Conference on Breakthroughs in Cancer Research: From Biology to Therapeutics
    • Place of Presentation
      Hyatt Regency Maui Maui, Hawaii
    • Year and Date
      2016-02-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of radiosensitizers targeting cancer stem cells in cervical cancer by using high throughput screening.2015

    • Author(s)
      Tamari K, Ishii H, Konno M, Nishida N, Kawamoto K, Koseki J, Beak SJ, Taketo K, Doki Y, Mori M, Ogawa K.
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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