| Project/Area Number |
26670573
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
Haga Sanae 北海道大学, 保健科学研究院, 特任講師 (60706505)
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| Co-Investigator(Kenkyū-buntansha) |
小澤 岳昌 東京大学, 大学院理学系研究科(理学部), 教授 (40302806)
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| Research Collaborator |
OZAKI Michitaka
MORITA Naoki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 光イメージング / 細胞生存 / 細胞療法 / 移植 / イメージング / 生存 |
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| Outline of Final Research Achievements |
In the present study, we tried to develop a new cell therapy system by using light-sensitive proteins to control cellular functions especially for cell survival.Akt is a key molecule for cell survival and protection against various stresses in many types of cells. Therefore, we developed the newly designed photo-activatable probe to control positively cellular Akt function (PA-Akt probe). PA-Akt which we made enabled cytosolic Akt to move to cell membrane by light illumination. With illumination blue light, PA-Akt successfully induced the activation (phosphorylation) of Akt and the downstream signals including GSK-3beta in hepatocytes. As a result, it conferred resistance hepatocytes against various stresses including oxidative stress and malnutrition.
Our cellular control system utilizing light-sensitive protein may provide a unique tool for the development of new therapies especially in the field of cell/organ transplantation.
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