Development of a new cell therapy system by optic control of cellular functions
Project/Area Number |
26670573
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General surgery
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Research Institution | Hokkaido University |
Principal Investigator |
Haga Sanae 北海道大学, 保健科学研究院, 特任講師 (60706505)
|
Co-Investigator(Kenkyū-buntansha) |
小澤 岳昌 東京大学, 大学院理学系研究科(理学部), 教授 (40302806)
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Research Collaborator |
OZAKI Michitaka
MORITA Naoki
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 光イメージング / 細胞生存 / 細胞療法 / 移植 / イメージング / 生存 |
Outline of Final Research Achievements |
In the present study, we tried to develop a new cell therapy system by using light-sensitive proteins to control cellular functions especially for cell survival.Akt is a key molecule for cell survival and protection against various stresses in many types of cells. Therefore, we developed the newly designed photo-activatable probe to control positively cellular Akt function (PA-Akt probe). PA-Akt which we made enabled cytosolic Akt to move to cell membrane by light illumination. With illumination blue light, PA-Akt successfully induced the activation (phosphorylation) of Akt and the downstream signals including GSK-3beta in hepatocytes. As a result, it conferred resistance hepatocytes against various stresses including oxidative stress and malnutrition. Our cellular control system utilizing light-sensitive protein may provide a unique tool for the development of new therapies especially in the field of cell/organ transplantation.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] p62/SQSTM1 Plays a Protective Role in Oxidative Injury of Steatotic Liver in a Mouse Hepatectomy Model.2014
Author(s)
Haga S, Ozawa T, Yamada Y, Morita N, Nagashima I, Inoue H, Inaba Y, Noda N, Abe R, Umezawa K, Ozaki M.
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Journal Title
Antioxid Redox Signal.
Volume: 21
Issue: 18
Pages: 2515-2530
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Anoikis induction and inhibition of peritoneal metastasis of pancreatic cancer cells by a nuclear factor-kappa B inhibitor, (-)-DHMEQ.2014
Author(s)
Masanori Sato, Kazuaki Nakanishi, Sanae Haga, Masato Fujiyoshi, Motoi Baba, Kazuhiro Mino, Yimin, Haruki Niwa, Hideki Yokoo, Kazuo Umezawa, Yoshihiro Ohmiya, Toshiya Kamiyama, Satoru Todo, Akinobu Taketomi, Michitaka Ozaki.
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Journal Title
Oncology Research
Volume: 21
Issue: 6
Pages: 333-343
DOI
Related Report
Peer Reviewed
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