| Project/Area Number |
26670584
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANIMIZU Naoki 札幌医科大学, 医学部, 准教授 (00333386)
ICHINOHE Norihisa 札幌医科大学, 医学部, 研究員 (80452978)
MIZUGUCHI Toru 札幌医科大学, 医学部, 准教授 (30347174)
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| Co-Investigator(Renkei-kenkyūsha) |
HIRATA Koichi 札幌医科大学, 医学部, 研究員 (50136959)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 小型肝細胞 / 肝前駆細胞 / 継代培養 / CD44 / Matrigel / 増殖 / self-renewal / 細胞外基質 / 細胞増殖 / laminin / 肝細胞機能 |
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| Outline of Final Research Achievements |
We performed the experiments to establish hepatocytic progenitor cell lines that can be subcultured. CD44-positive small hepatocytes (CD44-SHs) sorted from SH colonies were cultured in serum-free medium on Matrigel-coated dishes. CD44-SHs could be subcultured at least 4 times and maintain the growth ability for more than 4 months. The cells showed expression of hepatocyte-specific genes and proteins such as albumin and HNF4α in a whole period. CD44-SHs morphologically and functionally demonstrated typical characteristics as hepatocytes. When the cells were transplanted into NOG mouse livers, they could be engrafted and differentiate into hepatocytes. On the other hand, mouse SHs derived from a healthy adult mouse could be lined, which possessed hepatocytic characteristics. Although we succeeded in performing one passage of human SHs, we have not yet succeeded the sequential passages of human SHs. We try to establish human SH lines for clinical application in near future.
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