| Project/Area Number |
26670585
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKEBE Takanori 横浜市立大学, 医学部, 准教授 (20612625)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 再生医療 / 細胞・組織 / 再生医学 / 幹細胞 / 肝疾患 |
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| Outline of Final Research Achievements |
In this study, we evaluated feeder-free iPS cell culture condition for producing a knock- iPS cells, and evaluated optimal transfection conditions.Next, we established the knock-in iPS cells constitutively express a fluorescent protein. It was confirmed that the knock-in iPS cell clone expressed fluorescent protein even after differentiation induction. Furthermore, we succeeded to establish a knock-in iPS cells targeted for the hepatic differentiation marker gene. The established iPS cell could be useful to determine the optimal timing of hepatic cells for producing IPSC-liver bud toward clinical applications.
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