| Project/Area Number |
26670586
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Watanabe Taiji 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20448723)
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| Research Collaborator |
GOJO Satoshi 京都府立医科大学, 医学研究科, 教授 (90316745)
TABATA Yasuhiko 京都大学, 生体組織工学研究部門生体材料学分野, 教授 (50211371)
KAMI Daisuke 京都府立医科大学, 医学研究科, 講師 (80415588)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ミトコンドリア / 移植 / 心筋細胞 / 細胞内移植 / TATペプチド / 虚血再灌流障害 |
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| Outline of Final Research Achievements |
It is known that the efficiency of mitochondria transfer into cells in primary cultured cells is low efficiency. Therefore, we focusing on TAT peptide known as the cell membrane penetrating peptide, we have constructed a system that can high-efficiently transfer mitochondria into primary cultured cells. We introduced mitochondria using this TAT dextran and showed that the introduction efficiency of mitochondria improves on rat neonatal cardiomyocytes (RNCM), and it reduces the apoptosis induction rate of hydrogen peroxide treated RNCM. In the future, we will evaluate the mitochondrial function using the oxygen consumption rate as an indicator for mitochondrial activity of RNCM introduced mitochondria.
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