Basic study of the relevance of programmed cell death in the maintenance and regulation of liver function and regeneration for the future clinical use.
Project/Area Number |
26670591
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Hokkaido University |
Principal Investigator |
Yi Min 北海道大学, 医学(系)研究科(研究院), 助教 (40292007)
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Co-Investigator(Kenkyū-buntansha) |
HAGA Sanae 北海道大学, 保健科学研究院, 博士研究員 (60706505)
NODA Natusmi 東京大学, 理学研究科, 研究員 (30624358)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 肝障害 / プログラム細胞死 / 光プローブ / 再生 / 細胞死 / 機能維持 / イメージング / 昨日維持 |
Outline of Final Research Achievements |
Liver regeneration is an essential event in the treatment of various liver diseases. Most therapeutic strategies are based on the natural regenerative capability of liver. However, the precise mechanisms of liver regeneration have not fully elucidated. Here, we tried to study the roles of programmed cell death such as apoptosis, necroptosis and autophagy in post-hepatectomy liver regeneration. We designed and developed our original optic probes for apoptosis, necroptosis and autophagy (based on molecular mechanism of caspase, necroptosis and autophagy pathway, respectively). Some of these probes functioned at least hepatic cells in the in vitro system. We examined the functions of the apoptotic activity probe with Fas-induced acute liver failure model in mouse as well as cell experiments. The probe for apoptotic activity was proved to be sufficient for even in vivo assay. We hope to elucidate the physio-pathology of various liver conditions by these probes.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Gadd34 regulates liver regeneration in hepatic steatosis.2015
Author(s)
Yuka Inaba, Tomoko Furutani, Kumi Kimura, Hitoshi Watanabe, Sanae Haga, Yoshiaki Kido, Michihiro Matsumoto, Yasuhiko Yamamoto, Kenichi Harada, Shuichi Kaneko, Seiichi Oyadomari, Michitaka Ozaki, Masato Kasuga, Hiroshi Inoue.
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Journal Title
Hepatology
Volume: 61
Pages: 1343-1356
Related Report
Peer Reviewed
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[Journal Article] p62/SQSTM1 plays a protective role in oxidative injury of steatotic liver in a mouse hepatectomy model.2014
Author(s)
Sanae Haga, Takeaki Ozawa, Yuma Yamada, Naoki Morita, Izuru Nagashima, Hiroshi Inoue, Yuka Inaba, Natsumi Noda, Riichiro Abe, Kazuo Umezawa, Michitaka Ozaki.
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Journal Title
Antioxid Redox Signal
Volume: 21
Pages: 2515-2530
NAID
Related Report
Peer Reviewed
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[Journal Article] Anoikis induction and inhibition of peritoneal metastasis of pancreatic cancer cells by a nuclear factor-kappa B inhibitor, (-)-DHMEQ.2014
Author(s)
Masanori Sato, Kazuaki Nakanishi, Sanae Haga, Masato Fujiyoshi, Motoi Baba, Kazuhiro Mino, Yimin, Haruki Niwa, Hideki Yokoo, Kazuo Umezawa, Yoshihiro Ohmiya, Toshiya Kamiyama, Satoru Todo, Akinobu Taketomi, Michitaka Ozaki.
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Journal Title
Oncology Research
Volume: 21
Pages: 333-343
Related Report
Peer Reviewed
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