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Analysis on alterated chromatin status of viral genome in the host cells.

Research Project

Project/Area Number 26670595
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionChiba University

Principal Investigator

Kaneda Atsushi  千葉大学, 医学(系)研究科(研究院), 教授 (10313024)

Research Collaborator MATSUSAKA Keisuke  千葉大学, 医学研究院, 助教 (40610150)
FUNATA Sayaka  千葉大学, 医学研究院, 特任助教 (80756081)
OKABE Atsushi  千葉大学, 医学研究院, 特任助教 (80778118)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsエピゲノム
Outline of Final Research Achievements

While virus infection could induce epigenomic alterations in host and viral genomes, the alteration of epigenomic status of viral DNA in host cells is not fully understood. We here challenge clarification and visualization of epigenomic alteration of exogenous viral DNA in host cells. EBV-lacO plasmid DNA with oriP, the EBNA1 gene and lacO repeats, was transfected to 293T cells, and increase of DNA methylation levels was detected at day 50. When GFP-lacR and mCherry-MBD proteins were transfected, these proteins were co-localized in the nucleus, visualizing methylation status of episomal DNA in host cells. Next, a complex formation of methylated EBV-lacO and mCherry-MBD protein was observed under AFM, visualizing methylation status of DNA using AFM. Finally biotinylated EBV-lacO plasmids transfected to 293 cells were collected from the host cells, and the proteins interacting with the episomal DNA were detected by LC/MS/MS analysis, e.g. transcription factors and RNA-binding proteins.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (46 results)

All 2016 2015 2014 Other

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 9 results,  Acknowledgement Compliant: 2 results) Presentation (29 results) (of which Int'l Joint Research: 1 results,  Invited: 9 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Genetic and epigenetic aberrations occurring in colorectal tumors associated with serrated pathway.2016

    • Author(s)
      Sakai E, Fukuyo M, Ohata K, Matsusaka K, Doi N, Mano Y, Takane K, Abe H, Yagi K, Matsuhashi N, Fukushima J, Fukayama M, Akagi K, Aburatani H, Nakajima A, Kaneda A.
    • Journal Title

      Int J Cancer

      Volume: 138 Issue: 7 Pages: 1634-44

    • DOI

      10.1002/ijc.29903

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation.2016

    • Author(s)
      Ishige T, Nishimura M, Satoh M, Fujimoto M, Fukuyo M, Semba T, Kado S, Tsuchida S, Sawai S, Matsushita K, Togawa A, Matsubara H, Kaneda A, Nomura F
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 21681-21681

    • DOI

      10.1038/srep21681

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epigenetic alteration to activate Bmp2-Smad signaling in Raf-induced senescence.2016

    • Author(s)
      Fujimoto M, Mano Y, Anai M, Yamamoto S, Fukuyo M, Aburatani H, Kaneda A.
    • Journal Title

      World J Biol Chem.

      Volume: 7(1): Issue: 1 Pages: 188-205

    • DOI

      10.4331/wjbc.v7.i1.188

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hochu-ekki-to (Bu-zhong-yi-qi-tang), a herbal medicine, enhances cisplatin-induced apoptosis in HeLa cells.2015

    • Author(s)
      Sato T, Kita K, Sato C, Kaneda A.
    • Journal Title

      Mol Med Rep

      Volume: 2 Issue: 4 Pages: 6215-20

    • DOI

      10.3892/mmr.2015.4137

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epstein-Barr virus-associated gastric carcinoma: Use of host cell machineries and somatic gene mutations.2015

    • Author(s)
      Abe H, Kaneda A, Fukayama M.
    • Journal Title

      Pathobiology

      Volume: 82 Issue: 5 Pages: 212-232

    • DOI

      10.1159/000434683

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Quantitative DNA methylation analysis for epigenotyping of colorectal cancer.2015

    • Author(s)
      Kaneda A, Yagi K.
    • Journal Title

      Methods Mol Biol

      Volume: 1238 Pages: 289-99

    • DOI

      10.1007/978-1-4939-1804-1_15

    • ISBN
      9781493918034, 9781493918041
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spatial interplay between Polycomb and Trithorax complexes controls transcriptional activity in T lymphocytes.2015

    • Author(s)
      Onodera, A., Tumes, D. J., Watanabe, Y., Hirahara, K., Kaneda, A., Sugiyama, F., Suzuki, Y., and Nakayama, T.
    • Journal Title

      Mol. Cell. Biol.

      Volume: 35 Issue: 22 Pages: 3841-3853

    • DOI

      10.1128/mcb.00677-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] エピゲノム解析技法2015

    • Author(s)
      船田さやか、金田篤志.
    • Journal Title

      医学のあゆみ

      Volume: 255 Pages: 586-92

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 大腸癌に蓄積する分子異常と遺伝性大腸癌2015

    • Author(s)
      金田篤志、高根希世子.
    • Journal Title

      Jpn J Genet Counsel

      Volume: 36 Pages: 125-130

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Methylation epigenotypes and genetic features in colorectal laterally spreading tumors2014

    • Author(s)
      Sakai E, Ohata K, Chiba H, Matsuhashi N, Doi N, Fukushima J, Endo H, Takahashi H, Tsuji S, Yagi K, Matsusaka K, Aburatani H, Nakajima A, Kaneda A.
    • Journal Title

      Int J Cancer

      Volume: in press Issue: 7 Pages: 1586-95

    • DOI

      10.1002/ijc.28814

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aberrant promoter methylation of PPP1R3C and EFHD1 in plasma of colorectal cancer patients.2014

    • Author(s)
      Takane K, Midorikawa Y, Yagi K, Sakai A, Aburatani H, Takayama T, Kaneda A
    • Journal Title

      Cancer Med.

      Volume: 3 Issue: 5 Pages: 1235-45

    • DOI

      10.1002/cam4.273

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA methylation accumulation and its predetermination of future cancer phenotypes.2014

    • Author(s)
      Kaneda A, Matsusaka K, Sakai E, Funata S.
    • Journal Title

      J Biochem

      Volume: 156 Issue: 2 Pages: 63-72

    • DOI

      10.1093/jb/mvu038

    • NAID

      40020155022

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 胃癌におけるゲノム網羅的な解析から何が明らかになったか2014

    • Author(s)
      松坂恵介、金田篤志
    • Journal Title

      分子消化器病

      Volume: 11 Pages: 113-8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA methylation in gastric cancer, related to Helicobacter pylori and Epstein-Barr virus2014

    • Author(s)
      Keisuke Matsusaka, Sayaka Funata, Masashi Fukayama and Atsushi Kaneda.
    • Journal Title

      World Journal of Gastroenterology

      Volume: 20(14) Issue: 14 Pages: 3916-3926

    • DOI

      10.3748/wjg.v20.i14.3916

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Epstein-Barrウイルス感染による新規DNAメチル化とヒストン活性化マーク消失の相関2015

    • Author(s)
      船田さやか、松坂恵介、山中遼太、仲木竜、油谷浩幸、深山正久、金田篤志
    • Organizer
      第38回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ras/Raf誘導性細胞老化におけるNotchシグナルを介した癌防御機構の解析2015

    • Author(s)
      眞野恭伸、山中遼太、油谷浩幸、金田篤志
    • Organizer
      第38回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] エピソーマル型プラスミドベクターの長期培養後にみられるde novo DNAメチル化誘導の解析2015

    • Author(s)
      菅谷茂、松坂恵介、船田さやか、眞野恭伸、福世真樹、喜多和子、金田篤志
    • Organizer
      第38回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胃がんDNA異常メチル化に対する配列選択的DNA結合小分子を用いた新規抗がん療法の開発.2015

    • Author(s)
      松坂恵介、篠原憲一、福世真樹、金田篤志.
    • Organizer
      第1回AMEDがん若手研究者ワークショップ
    • Place of Presentation
      国立オリンピック記念青少年総合センター(東京都・渋谷区)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] DNAメチル化情報による遺伝性大腸癌の層別化2015

    • Author(s)
      高根希世子、松坂恵介、太田聡、酒井英嗣、福世真樹、松下一之、宮内英聡、油谷浩幸、中谷行雄、高山忠利、松原久裕、金田篤志.
    • Organizer
      第1回AMEDがん若手研究者ワークショップ
    • Place of Presentation
      国立オリンピック記念青少年総合センター(東京都・渋谷区)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胃がんにおいてEBV感染がもたらすDNAメチル化・ヒストン修飾変化2015

    • Author(s)
      船田さやか、松坂恵介、山中遼太、仲木竜、油谷浩幸、深山正久、金田篤志.
    • Organizer
      第1回AMEDがん若手研究者ワークショップ
    • Place of Presentation
      国立オリンピック記念青少年総合センター(東京都・渋谷区)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 消化器癌に蓄積するエピゲノム異常の意義2015

    • Author(s)
      金田篤志
    • Organizer
      埼玉東部バイオマーカーセミナー
    • Place of Presentation
      獨協医科大学越谷病院(埼玉県・越谷市)
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Molecular basis of colorectal tumors develped through serrated pathway.2015

    • Author(s)
      Eiji Sakai, Atsushi Kaneda, Atsushi Nakajima.
    • Organizer
      United European Gastroentorelogy Week 2015
    • Place of Presentation
      Barcelona (Spain)
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Four DNA methylation epigenotypes of gastric cancer with distinct etiology and genetic features, revealed by genome-wide analyses.2015

    • Author(s)
      Atsushi Kaneda, Keisuke Matsusaka, Sayaka Funata, Masaki Fukuyo, Hiroe Namba-Fukuyo, Masayuki Urabe, Masashi Fukayama.
    • Organizer
      74th Annual Meeting of Japan Cancer Association
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dynamic alteration of distribution of hydroxymethylcytosine during DNA methylation alteration by EB virus infection.2015

    • Author(s)
      Hiroe N Fukuyo, Masaki Fukuyo, Keisuke Matsusaka, Sayaka Funata, Masashi Fukayama, Toshiki Watanabe, Atsushi Kaneda.
    • Organizer
      74th Annual Meeting of Japan Cancer Association.
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Genetic aberration analysis combined with DNA methylome characterization in gastric cancer.2015

    • Author(s)
      Keisuke Matsusaka, Masaki Fukuyo, Masayuki Urabe, Bahityar Rahmutulla, Eiji Sakai, Hiroyuki Aburatani, Masashi Fukayama, Atsushi Kaneda.
    • Organizer
      74th Annual Meeting of Japan Cancer Association
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Two subtypes with distinct molecular features in colorectal neoplasms of familial adenomatous polyposis.2015

    • Author(s)
      Kiyoko Takane, Keisuke Matsusaka, Satoshi Ota, Eiji Sakai, Masaki Fukuyo, Kazuyuki Matsushita, Hideaki Miyauchi, Hiroyuki Aburatani, Yukio Nakatani, Tadatoshi Takayama, Hisahiro Matsubara, Atsushi Kaneda.
    • Organizer
      74th Annual Meeting of Japan Cancer Association
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization of DNA methylation abnormalities in esophagogastric junction adenocarcinoma and background epithelium.2015

    • Author(s)
      Masayuki Urabe, Keisuke Matsusaka, Tetsuo Ushiku, Masaki Fukuyo, Hiroharu Yamashita, Yasuyuki Seto, Masashi Fukayama, Atsushi Kaneda.
    • Organizer
      74th Annual Meeting of Japan Cancer Association
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular Basis of Colorectal Tumors Developed Through Serrated Pathway2015

    • Author(s)
      酒井英嗣,金田篤志,中島淳.
    • Organizer
      JDDW 2015
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都・港区)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] エピジェネティクスとがん進化2015

    • Author(s)
      金田篤志
    • Organizer
      日本進化学会第17回大会
    • Place of Presentation
      中央大学後楽園キャンパス(東京都・文京区)
    • Year and Date
      2015-08-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ゲノムとエピゲノム~その異常と癌~2015

    • Author(s)
      金田篤志
    • Organizer
      千葉医学会第91回学術大会
    • Place of Presentation
      ゐのはな記念講堂(千葉県・千葉市)
    • Year and Date
      2015-07-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] DNAメチル化情報による遺伝性大腸癌の層別化2015

    • Author(s)
      高根希世子、松坂恵介、太田聡、酒井英嗣、福世真樹、松下一之、宮内英聡、油谷浩幸、中谷行雄、高山忠利、松原久裕、金田篤志
    • Organizer
      第70回日本消化器外科学会総会
    • Place of Presentation
      アクトシティ浜松(静岡県・浜松市)
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸発癌に重要な分子異常と遺伝素因2015

    • Author(s)
      金田篤志
    • Organizer
      第39回日本遺伝カウンセリング学会学術集会
    • Place of Presentation
      三井ガーデンホテル千葉(千葉県・千葉市)
    • Year and Date
      2015-06-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 消化管腫瘍を誘導するエピジェネティクス異常2015

    • Author(s)
      金田篤志
    • Organizer
      第9回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都・千代田区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] DNAメチル化マーカーおよび癌遺伝子パネル解析を用いた大腸LSTの分子生物学的背景の解明2015

    • Author(s)
      酒井英嗣,金田篤志,中島淳
    • Organizer
      第101回消化器病学会総会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Epstein-Barr virus infection is an epigenomic driver of gastric tumorigenesis2015

    • Author(s)
      Kaneda A
    • Organizer
      Japan-German Workshop
    • Place of Presentation
      京都ロイヤルホテル(京都府・京都市)
    • Year and Date
      2015-03-01
    • Related Report
      2014 Research-status Report
  • [Presentation] エピゲノム異常特性に基づいた発癌機構の解明2015

    • Author(s)
      金田篤志
    • Organizer
      Takeda Genome Urology 2014
    • Place of Presentation
      シェラトン都ホテル東京(東京都・港区)
    • Year and Date
      2015-02-07
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ホスト細胞内における外来DNAのエピゲノム状態の可視化2014

    • Author(s)
      菅谷茂, 松坂恵介, 船田さやか, 喜多和子, 金田篤志
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Comprehensive analysis of the cancer genome and epigenome to clarify and manage cancer2014

    • Author(s)
      Kaneda A
    • Organizer
      11th JGFoS Symposium
    • Place of Presentation
      Bremen (Germany)
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] DNA methylation accumulation at early stage of carcinogenesis, predetermining future cancer phenotypes2014

    • Author(s)
      Atsushi Kaneda, Keisuke Matsusaka, Eiji Sakai, Koichi Yagi, Sayaka Funata, Hiroyuki Aburatani, Atsushi Nakajima, Masashi Fukayama
    • Organizer
      73rd Annual Meeting of Japan Cancer Association
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Epstein-Barr virus infection induces de novo DNA methylation in non-cancerous gastric epithelial cells.2014

    • Author(s)
      Keisuke Matsusaka, Sayaka Funata, Hiroyuki Aburatani, Masashi Fukayama, Atsushi Kaneda.
    • Organizer
      73rd Annual Meeting of Japan Cancer Association
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Inverse correlation between active histone marks and induction of DNA methylation during Epstein-Barr virus infection2014

    • Author(s)
      Sayaka Funata, Keisuke Matsusaka, Ryota Yamanaka, Hiroyuki Aburatani, Masashi Fukayama、 Atsushi Kaneda
    • Organizer
      73rd Annual Meeting of Japan Cancer Association
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Epigenetic alteration accumulated in gastrointestinal carcinogenesis2014

    • Author(s)
      Kaneda A
    • Organizer
      RIKEN IMS Summer Program 2014
    • Place of Presentation
      理化学研究所(神奈川県・横浜市)
    • Year and Date
      2014-06-20
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] エピゲノム異常と消化器発癌2014

    • Author(s)
      金田篤志
    • Organizer
      第15回 京都消化器カンファランス
    • Place of Presentation
      芝蘭会館(京都府・京都市)
    • Year and Date
      2014-04-01
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] 「質量分析やビーズアレイによる網羅的解析」in 「エピジェネティクス―基礎研究から産業応用への展望―」2015

    • Author(s)
      船田さやか、金田篤志
    • Publisher
      シーエムシー出版
    • Related Report
      2014 Research-status Report
  • [Book] 「エピジェネティクスの疾患」in 「動物の事典」2015

    • Author(s)
      金田篤志
    • Publisher
      朝倉書店
    • Related Report
      2014 Research-status Report
  • [Remarks] 千葉大学大学院医学研究院分子腫瘍学

    • URL

      http://www.m.chiba-u.ac.jp/class/moloncol/index.html

    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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