Analysis on alterated chromatin status of viral genome in the host cells.
Project/Area Number |
26670595
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Chiba University |
Principal Investigator |
Kaneda Atsushi 千葉大学, 医学(系)研究科(研究院), 教授 (10313024)
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Research Collaborator |
MATSUSAKA Keisuke 千葉大学, 医学研究院, 助教 (40610150)
FUNATA Sayaka 千葉大学, 医学研究院, 特任助教 (80756081)
OKABE Atsushi 千葉大学, 医学研究院, 特任助教 (80778118)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | エピゲノム |
Outline of Final Research Achievements |
While virus infection could induce epigenomic alterations in host and viral genomes, the alteration of epigenomic status of viral DNA in host cells is not fully understood. We here challenge clarification and visualization of epigenomic alteration of exogenous viral DNA in host cells. EBV-lacO plasmid DNA with oriP, the EBNA1 gene and lacO repeats, was transfected to 293T cells, and increase of DNA methylation levels was detected at day 50. When GFP-lacR and mCherry-MBD proteins were transfected, these proteins were co-localized in the nucleus, visualizing methylation status of episomal DNA in host cells. Next, a complex formation of methylated EBV-lacO and mCherry-MBD protein was observed under AFM, visualizing methylation status of DNA using AFM. Finally biotinylated EBV-lacO plasmids transfected to 293 cells were collected from the host cells, and the proteins interacting with the episomal DNA were detected by LC/MS/MS analysis, e.g. transcription factors and RNA-binding proteins.
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Report
(3 results)
Research Products
(46 results)
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[Journal Article] Genetic and epigenetic aberrations occurring in colorectal tumors associated with serrated pathway.2016
Author(s)
Sakai E, Fukuyo M, Ohata K, Matsusaka K, Doi N, Mano Y, Takane K, Abe H, Yagi K, Matsuhashi N, Fukushima J, Fukayama M, Akagi K, Aburatani H, Nakajima A, Kaneda A.
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Journal Title
Int J Cancer
Volume: 138
Issue: 7
Pages: 1634-44
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation.2016
Author(s)
Ishige T, Nishimura M, Satoh M, Fujimoto M, Fukuyo M, Semba T, Kado S, Tsuchida S, Sawai S, Matsushita K, Togawa A, Matsubara H, Kaneda A, Nomura F
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 21681-21681
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Spatial interplay between Polycomb and Trithorax complexes controls transcriptional activity in T lymphocytes.2015
Author(s)
Onodera, A., Tumes, D. J., Watanabe, Y., Hirahara, K., Kaneda, A., Sugiyama, F., Suzuki, Y., and Nakayama, T.
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Journal Title
Mol. Cell. Biol.
Volume: 35
Issue: 22
Pages: 3841-3853
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Methylation epigenotypes and genetic features in colorectal laterally spreading tumors2014
Author(s)
Sakai E, Ohata K, Chiba H, Matsuhashi N, Doi N, Fukushima J, Endo H, Takahashi H, Tsuji S, Yagi K, Matsusaka K, Aburatani H, Nakajima A, Kaneda A.
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Journal Title
Int J Cancer
Volume: in press
Issue: 7
Pages: 1586-95
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Two subtypes with distinct molecular features in colorectal neoplasms of familial adenomatous polyposis.2015
Author(s)
Kiyoko Takane, Keisuke Matsusaka, Satoshi Ota, Eiji Sakai, Masaki Fukuyo, Kazuyuki Matsushita, Hideaki Miyauchi, Hiroyuki Aburatani, Yukio Nakatani, Tadatoshi Takayama, Hisahiro Matsubara, Atsushi Kaneda.
Organizer
74th Annual Meeting of Japan Cancer Association
Place of Presentation
名古屋国際会議場(愛知県・名古屋市)
Year and Date
2015-10-08
Related Report
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