Development of the effect predictive diagnostic method by a Her2 antibody conjugated liposomal ICG before treatment

• Project/Area Number: 26670596
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: Hoshino Isamu 千葉大学, 医学(系)研究科(研究院), 特任研究員 (10400904)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 胃癌 / 不均一性 / ICG / ナノ粒子 / 腹膜播種 / HER2 / リンパ節転移 / ナノシリカ粒子

Outline of Final Research Achievements

The purpose of this study is developing a new diagnostic method to evaluate the effectiveness of the antibody treatment in digestive cancer with heterogeneity. We evaluated Her2 expression in primary tumor or the metastasis lymph node and we confirmed the heterogeneity in gastric cancer. We recognized a difference Her2 expression pattern between the primary lesion and the lymph node in six of ten cases. LP-ICG-C18 was made the liposome that can be observed with fluorescence imaging system. The identification of tumor including LP-ICG-C18 was easy by using near-infrared fluorescence imaging system. Furthermore, we confirmed the antigen-antibody reaction with Her2-LP-ICG-C18. We established Quantum Dots Conjugated with Monoclonal　Anti-HER2 Antibody (ICG-QD), and the signal which was more powerful. It was thought that it became the base to a diagnosis in the preoperation of Her2-positive tumor using this probe.

Research Products

• [Journal Article] Detection of peritoneal dissemination with near-infrared fluorescence laparoscopic imaging using a liposomal formulation of a synthesized indocyanine green liposomal derivative. (2015)
  • Author(s): Hoshino I, Maruyama T, Fujito H, Tamura Y, Suganami A, Hayashi H, Toyota T, Akutsu Y, Murakami K, Isozaki Y, Akanuma N, Takeshita N, Toyozumi T, Komatsu A, Matsubara H.
  • Journal Title: Anticancer Res. Volume: 35 Pages: 1363-1359
  • Peer Reviewed
• [Journal Article] Treatment of near-infrared photodynamic therapy using a liposomally formulated indocyanine green derivative for squamous cell carcinoma (2015)
  • Author(s): Maruyama T, Akutsu Y, Suganami A, Tamura Y, Fujito H, Ouchi T, Akanuma N, Isozaki Y, Takeshita N, Hoshino I, Uesato M, Toyota T, Hayashi H, Matsubara H
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0122849-e0122849
  • DOI: 10.1371/journal.pone.0122849
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of Peritoneal Dissemination with Near-infrared Fluorescence Laparoscopic Imaging Using a Liposomal Formulation of a Synthesized Indocyanine Green Liposomal Derivative. (2015)
  • Author(s): Hoshino I, Maruyama T, Fujito H, Tamura Y, Suganami A, Hayashi H, Toyota T, Akutsu Y, Murakami K, Isozaki Y, Akanuma N, Takeshita N, Toyozumi T, Komatsu A, Matsubara H.
  • Journal Title: Anticancer Res. Volume: 35 Pages: 1353-1359
  • Peer Reviewed
• [Presentation] リポソーム化ICGを用いた審査腹腔鏡の有用性の検討 (2014)
  • Author(s): 星野 敢
  • Organizer: 第69回日本消化器外科学会総会
  • Place of Presentation: 郡山市民文化センター （福島県、郡山市）
  • Year and Date: 2014-07-17 – 2014-07-19