Biomarker of the colorectal cancer in inflammatory bowel decease patients.

• Project/Area Number: 26670598
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: The University of Tokyo
• Principal Investigator: SUNAMI EIJI 東京大学, 医学部附属病院, 登録研究員 (70345205)
• Co-Investigator(Kenkyū-buntansha): ISHIHARA SOICHIRO 東京大学, 医学部付属病院, 講師 (00376443) ; YAMAGUCHI HIRONORI 東京大学, 医学部付属病院, 登録研究員 (20376445) ; WATANABE TOSHIAKI 東京大学, 医学部付属病院, 教授 (80210920)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 炎症性腸疾患 / 大腸がん / バイオマーカー / 遊離DNA / LINE1 / サーベイランス

Outline of Final Research Achievements

Inflammatory Bowel decease (IBD) may cause colorectal cancer after long disease duration. In this study, the possibility of early detection of the colorectal cancer by means of the blood, feces and biopsy is investigated. In this study, we quantified the global methylation status of cfDNA in plasma extracted from colorectal cancer (CRC) patients, and investigated its clinical usefulness as a biomarker. LINE-1 hypomethylation index (LHI) in plasma may have a potential to serve as a biomarker enabling us to detect CRC patient at early stage. By means of the biopsy specimen, early onset of LINE-1 demethylation during adenomatous change of colorectal epithelial cells and LINE-1 demethylation progression is linear to TNM stage progression are demonstrated. LINE-1 hypomethylation is also detected from the material of the feces and regarding the utilization of this specimen, further analyses of the data are necessary.

Research Products

• [Journal Article] Incidence of neoplasias and effectiveness of post operative surveillance endoscopy for patients with ulcerative colitis: comparison of ileorectal anastomosis and ileal pouch-anal anastomosis. (2016)
  • Author(s): Ishii H, Hata K, Kishikawa J, Anzai H, OtaniK, Yasuda K, Nishikawa T, Tanaka T, Tanaka J Kiyomatsu T, Kawai K, Nozawa H, Kazama S, Yamaguchi H, Ishihara S, Sunami E, Kitayama j, Watanabe T
  • Journal Title: World Journal of Surgical Oncology Volume: 14 Issue: 1 Pages: 833-835
  • DOI: 10.1186/s12957-016-0833-5
  • Peer Reviewed / Open Access
• [Journal Article] Surveillance colonoscopy for colitis-associated dysplasia and cancer in ulcerative colitis patients. (2015)
  • Author(s): Hata K, Kishikawa J, Anzai H, Shinagawa T, Kazama S, Ishii H, Nozawa H, Kawai K, Kiyomatsu T, Tanaka J, Tanaka T, Nishikawa T, Otani K, Yasuda K, Yamaguchi H, Ishihara S, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Dig Endosc Volume: 28 Issue: 3 Pages: 260-5
  • DOI: 10.1111/den.12505
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tracheobronchitis with Dyspnea in a Patient with Ulcerative Colitis (2015)
  • Author(s): Hiyoshi M, Kawai K, Shibuya M, Ozawa T, Kishikawa J, Nirei T, Tanaka T, Tanaka J, Kiyomatsu T, Tada T, Kanazawa T, Kazama S, Shoda H, Sumitomo S, Kubo K, Yamaguchi H, Ishihara S, Sunami E, Kitayama J, Yamamoto K, Watanabe T.
  • Journal Title: Internal Medicine Volume: 54 Issue: 7 Pages: 749-753
  • DOI: 10.2169/internalmedicine.54.3142
  • NAID: 130005063203
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Laparoscopic surgery for ulcerative colitis: a review of the literature. (2015)
  • Author(s): Hata K, Kazama S, Nozawa H, Kawai K, Kiyomatsu T, Tanaka J, Tanaka T, Nishikawa T, Yamaguchi H, Ishihara S, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Surg Today Volume: Epub ahead of print
  • Peer Reviewed
• [Journal Article] The laser capture microdissection cannot be replaced with the macrodissection. (2014)
  • Author(s): Sunami E, Watanabe T.
  • Journal Title: Exp Mol Patho Volume: 96 Issue: 2 Pages: 206-206
  • DOI: 10.1016/j.yexmp.2014.01.003
  • Peer Reviewed
• [Presentation] LINE-1 hypomethylation of circulating cell-free DNA in plasma has a potential to serve as a biomarker for detecting colorectal cancer patient (2015)
  • Author(s): Yuzo Nagai, Eiji Sunami, Yoko Yamamoto, Koji Yasuda, Kensuke Otani, Takeshi Nishikawa, Toshiaki Tanaka, Junichiro Tanaka, Tomomichi Kiyomatsu, Keisuke Hata, Kazushige Kawai, Hiroaki Nozawa, Hironori Yamaguchi, Soichiro Ishihara, Joji Kitayama, Toshiaki Watanabe
  • Organizer: 第70回日本消化器外科学会総会
  • Place of Presentation: アクトシティ浜松（静岡県・浜松市）
  • Year and Date: 2015-07-15