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Development of the early diagnostic method and the new nucleic acid treatment using exosome

Research Project

Project/Area Number 26670602
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionOsaka University

Principal Investigator

Tamai Koki  大阪大学, 医学部附属病院, 医員 (60724250)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords膵癌 / エクソソーム / microRNA / microRNA(miRNA) / 早期診断
Outline of Final Research Achievements

The stable isolation method of exosomes from human plasma was established in our laboratory as the first step. We employed the exosome isolation kit for the isolation because of the validated stable results. Based on the results of microarray assuming the expression in exosome from anti-cancer drug sensitive/resistant cancer cell lines, several miRNAs were picked up as the factor regarding the drug resistance. We validated the candidate miRNAs, and confirmed a certain miRNA was related with drug resistance via anti-apoptosis effect in vitro. The expression levels of exosomal candidate miRNA were significantly related to the patient’s disease-free survival time, and the expression would be a hopeful biomarker in clinical course. Now, we are working on the investigation of the feasibility about the drug delivery system in vitro. The exosome containing the certain miRNA deliver the dramatic effect of anti-apoptosis into the recipient cells in preliminary study.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Presentation (4 results)

  • [Presentation] The role of pancreatic cancer-derived exosome in cancer microenvironment under hypoxic condition2015

    • Author(s)
      三賀森 学
    • Organizer
      第70回日本消化器外科学会
    • Place of Presentation
      浜松
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膵癌細胞株におけるエクソソームを介した薬剤耐性獲得機構の証明2015

    • Author(s)
      三賀森 学
    • Organizer
      第115回日本外科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膵癌細胞株におけるエクソソームを介した薬剤耐性獲得機構の解明2014

    • Author(s)
      三賀森 学
    • Organizer
      第115回日本外科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-04-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 低酸素下における膵癌細胞由来エクソソームが癌細胞の悪性度に与える影響2014

    • Author(s)
      梶原 淳
    • Organizer
      第114回日本外科学会
    • Place of Presentation
      京都
    • Year and Date
      2014-04-05
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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