| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
MicroRNA (miR) is an attractive modulator in the field of cancer research, and it is known that its aberrant expression causes dysregulation of cancer-related genes. We previously reported that introduction of miRs (miR-200c, 302, 369) in colon cancer cells displayed an ability to change epigenetic profiles to the pluripotent state, and inhibited malignant features, including cell proliferation, chemoresistance, and tumorigenicity. In this study, we found that administration of the miRs suppressed the incidence of tumors in vivo in the course of tumorigenesis of CPC/Apc mouse, a mouse model of adenoma-carcinoma progression. In addition, these miRs increased expression of MAF gene, an inducer of apoptosis-related gene p53 in the normal mucosa. In vitro experiments supported the hypothesis that MAF behaved as a tumor suppressor in the p53-dependent mechanism.
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