Role of epigenetic modification by cancer reprograming in colon tumorigenesis model
Project/Area Number |
26670605
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
|
Research Institution | Osaka University |
Principal Investigator |
Yamamoto Hirofumi 大阪大学, 医学(系)研究科(研究院), 教授 (30322184)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | microRNA / miR-200c / miR-369 / miR-302 / CPC/Apc mouse / MAF / P53 / APC遺伝子 / 大腸癌 / マイクロRNA / リプログラム / CPC-Apcマウス / リプログラミング / エピゲノム |
Outline of Final Research Achievements |
MicroRNA (miR) is an attractive modulator in the field of cancer research, and it is known that its aberrant expression causes dysregulation of cancer-related genes. We previously reported that introduction of miRs (miR-200c, 302, 369) in colon cancer cells displayed an ability to change epigenetic profiles to the pluripotent state, and inhibited malignant features, including cell proliferation, chemoresistance, and tumorigenicity. In this study, we found that administration of the miRs suppressed the incidence of tumors in vivo in the course of tumorigenesis of CPC/Apc mouse, a mouse model of adenoma-carcinoma progression. In addition, these miRs increased expression of MAF gene, an inducer of apoptosis-related gene p53 in the normal mucosa. In vitro experiments supported the hypothesis that MAF behaved as a tumor suppressor in the p53-dependent mechanism.
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Report
(3 results)
Research Products
(1 results)
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[Presentation] Epigenetic alterations by microRNAs in carcinogenesis of colorectal cancer2016
Author(s)
Tsuyoshi Hata, Hirofumi Yamamoto, Daisuke Okuzaki, Hisataka Ogawa, Konno Msamitsu, Hidekazu Takahashi, Naoaki Haraguchi, Junichi Nishimura, Taishi Hata, Ichiro Takemasa, Taro Sato, Tsunekazu Mizushima, Hideshi Ishii, Yuichiro Doki, Masaki Mori
Organizer
69th Annual Cancer Symposium of the Society of Surgical Oncology (SSO 2016)
Place of Presentation
米国, マサチューセッツ州, ボストン
Year and Date
2016-03-03
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