High sensitive mutation analysis to identify early-stage pancreatic cancer
Project/Area Number |
26670613
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | National Cancer Center Japan |
Principal Investigator |
Yachida Shinichi 国立研究開発法人国立がん研究センター, 研究所がんゲノミクス研究分野, ユニット長 (20359920)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 膵臓がん |
Outline of Final Research Achievements |
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. It is known that >90% of PDAC harbor mutations in the KRAS gene as founder mutations. Circulating cell-free DNA (cfDNA) is a promising resource to detect and monitor molecular characteristics of tumors. In the present study, we determined the mutational status of KRAS in plasma cfDNA using multiplex picoliter-droplet digital PCR in 259 patients with PDAC. Two hundred and fifty-nine PDAC patients were involved in this study. Among 151 inoperable patients, mutant KRAS was detected in 63 of 107 (58.9%) PDAC patients with distant organ metastasis. The presence of ctDNA was significantly (P < 0.0001) associated with the presence of distant organ metastasis. Mutated KRAS genes could not be detected in the early stage of PDAC, so it would be premature to recommend the current protocol for screening to detect early-stage PDAC.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Clinical utility of circulating tumor DNA for molecular assessment in pancreatic cancer2015
Author(s)
Erina Takai, Yasushi Totoki, Hiromi Nakamura, Chigusa Morizane, Satoshi Nara, Natsuko Hama, Masami Suzuki, Eisaku Furukawa, Mamoru Kato, Hideyuki Hayashi, Takashi Kohno, Hideki Ueno, Kazuaki Shimada, Takuji Okusaka, Hitoshi Nakagama, Tatsuhiro Shibata, and Shinichi Yachida
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 18425-18425
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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