| Project/Area Number |
26670643
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 疼痛 / 神経 / 神経科学 |
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| Outline of Final Research Achievements |
We demonstrate that Netrin-4, a member of the axon guidance molecule family, was expressed in dorsal horn inner lamina II excitatory interneurons of rat spinal cord. A similar expression pattern for Netrin-4 was also observed in human spinal cord. Behavioral analysis revealed that Netrin-4 mutant rats showed abolishment of tactile and heat hyperalgesia that occurs after peripheral nerve injury or inflammation. Transient suppression of Netrin-4, or its receptor Unc5B, after injury could prevent allodynia. Conversely, intrathecal Netrin-4 protein administration to naiive rats enhanced excitatory synaptic transmission in the dorsal horn and induced allodynia, suggesting Netrin-4 is involved in spinal sensitization. Furthermore, Unc5B receptor and activation of tyrosine phosphatase SHP2 mediated Netrin-4-induced pain signal in the spinal cord.
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