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Maintenance of homeostasis for intervertebral disc by cell cycle-related genes

Research Project

Project/Area Number 26670651
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionHokkaido University

Principal Investigator

Sudo Hideki  北海道大学, 医学(系)研究科(研究院), 准教授 (30374367)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords椎間板変性 / 細胞周期 / 細胞周期関連遺伝子
Outline of Final Research Achievements

Although human intervertebral disc (IVD) degeneration can cause several spinal diseases, its pathogenesis remains unclear. This study aimed to create a highly reproducible in vivo mouse IVD degeneration model. One hundred six mice were operated and L4/5 IVD was stabbed with a 35 or 33-gauge needle. Micro-computed tomography scanning was performed, and the stabbed region was confirmed. Evaluation was by magnetic resonance imaging (MRI) and histology using our classification scoring system. In IVDs stabbed with either needle, significant degeneration occurred relative to the control. Our histological classification scores correlated well with MRI findings and could detect degenerative progression, irrespective of the stabbed region.In this study, a highly reproducible in vivo mouse IVD degeneration model was created with precise information about surgical techniques.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Surgical treatment of Lenke 1 thoracic adolescent idiopathic scoliosis with maintenance of kyphosis using the simultaneous double-rod rotation technique2014

    • Author(s)
      Sudo H,Ito M, Abe Y,Abumi K,Takahata M,Nagahama K, Hiratsuka S,Kuroki K,Iwasaki N
    • Journal Title

      Spine

      Volume: 39 Issue: 14 Pages: 1163-69

    • DOI

      10.1097/brs.0000000000000364

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of single injection of local anesthetic agents on intervertebral disc degeneration: ex vivo and long-term in vivo experimental study2014

    • Author(s)
      Iwasaki K, Sudo H, Yamada K, Higashi H, Ohnishi T, Tsujimoto T, Iwasaki N.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 10 Pages: e109851-e109851

    • DOI

      10.1371/journal.pone.0109851

    • NAID

      120005527839

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 遺伝子改変マウスを用いた椎間板変性におけるcaspase 3遺伝子の果たす機能解析2016

    • Author(s)
      大西貴士, 須藤英毅, 岩崎浩司, 辻本武尊, 岩崎倫政
    • Organizer
      日本脊椎脊髄病学会
    • Place of Presentation
      幕張メッセ国際会議場、千葉県
    • Year and Date
      2016-04-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新病理分類に基づいた再現性の高いin vivoマウス腰椎椎間板変性モデルの確立2016

    • Author(s)
      大西貴士, 須藤英毅, 岩崎浩司, 辻本武尊, 岩崎倫政
    • Organizer
      日本脊椎脊髄病学会
    • Place of Presentation
      幕張メッセ国際会議場、千葉県
    • Year and Date
      2016-04-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Highly reproducible in vivo mouse intervertebral disc degeneration model based on newly developed histological classification2016

    • Author(s)
      Takashi Ohnishi, Hideki Sudo, Takeru Tsujimoto, Norimasa Iwasaki
    • Organizer
      Orthopaedic Research Society
    • Place of Presentation
      Orlando, FL,USA
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 再現性の高いマウス椎間板穿刺変性モデルの作成2015

    • Author(s)
      大西貴士,須藤英毅,岩崎浩司,辻本武尊,岩崎倫政
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      富山国際会議場、富山県
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 再現性の高いマウス椎間板穿刺変性モデルの作成2015

    • Author(s)
      大西貴士,須藤英毅,岩崎倫政
    • Organizer
      北海道整形災害外科学会
    • Place of Presentation
      札幌医科大学(北海道札幌市)
    • Year and Date
      2015-01-31 – 2015-02-01
    • Related Report
      2014 Research-status Report
  • [Presentation] 思春期特発性胸椎側弯症(Lenke type 1)に対する3次元的後方矯正固定術の臨床成績2014

    • Author(s)
      須藤英毅,伊東学,安倍雄一郎,鐙邦芳,高畑雅彦,長濱賢,平塚重人,黒木圭,岩崎倫政
    • Organizer
      第87回日本整形外科学会学術集会
    • Place of Presentation
      神戸ポートピアホテル(兵庫県神戸市)
    • Year and Date
      2014-05-22 – 2014-05-25
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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