| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Although human intervertebral disc (IVD) degeneration can cause several spinal diseases, its pathogenesis remains unclear. This study aimed to create a highly reproducible in vivo mouse IVD degeneration model. One hundred six mice were operated and L4/5 IVD was stabbed with a 35 or 33-gauge needle. Micro-computed tomography scanning was performed, and the stabbed region was confirmed. Evaluation was by magnetic resonance imaging (MRI) and histology using our classification scoring system. In IVDs stabbed with either needle, significant degeneration occurred relative to the control. Our histological classification scores correlated well with MRI findings and could detect degenerative progression, irrespective of the stabbed region.In this study, a highly reproducible in vivo mouse IVD degeneration model was created with precise information about surgical techniques.
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