Identification of functional glyco-biomarkers during chondrogenesis of mouse iPS cells

• Project/Area Number: 26670652
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: Iwasaki Norimasa 北海道大学, 医学(系)研究科(研究院), 教授 (30322803)
• Co-Investigator(Kenkyū-buntansha): ONODERA Tomohiro 北海大学, 大学病院, 講師 (70547174)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 幹細胞 / 軟骨細胞分化 / 糖鎖 / 軟骨代謝 / iPS細胞 / 分化マーカー

Outline of Final Research Achievements

Alterations in the structure of stem cell surface glycoforms were found during chondrogenesis. Based on the obtained results, several glycans are considered to play important roles in stem cell-derived chondrogenesis. The identified glycans have functional potential of chondrogenic biomarkers for the safety of cartilage regenerative medicine.

Research Products

• [Journal Article] Chondrogenic Differentiation Of Mouse Induced Pluripotent Stem Cells (iPSCs) Using The Three Dimensional Culture With Ultra-purified Alginate Gel (UPAL Gel) (2016)
  • Author(s): Hontani K, Onodera T, Momma D, Matsuoka M, Baba R, Joutoku Z, Matsubara S, Homan K, Hishimura R, Kim WY, Iwasaki N
  • Journal Title: ORS Transaction Volume: 41 Pages: 1376-1376
  • Peer Reviewed
• [Presentation] Chondrogenic Differentiation Of Mouse Induced Pluripotent Stem Cells (iPSCs) Using The Three Dimensional Culture With Ultra-purified Alginate Gel (UPAL Gel) (2016)
  • Author(s): Hontani K, Onodera T, Momma D, Matsuoka M, Baba R, Joutoku Z, Matsubara S, Homan K, Hishimura R, Kim WY, Iwasaki N
  • Organizer: ORS 2016 Annual Meeting
  • Place of Presentation: Orland,FL,USA
  • Year and Date: 2016-03-05
  • Int'l Joint Research
• [Presentation] Ultra-purified alginate gel(UPAL gel)と多段階分化誘導法を併用したマウスiPS細胞の生体内における軟骨分化誘導 (2016)
  • Author(s): 本谷和俊、小野寺智洋、寺島理代、門間太輔、松岡正剛、馬場力哉、上徳善太、松原新史、宝満健太郎、菱村亮介、岩崎倫政
  • Organizer: 第29回 日本軟骨代謝学会
  • Place of Presentation: 広島
  • Year and Date: 2016-02-19
• [Presentation] 軟骨再生医学の臨床応用 －基礎から臨床へ向けてー (2015)
  • Author(s): 岩崎倫政
  • Organizer: Sapporo Orthopaedic Seminar 2015
  • Place of Presentation: 札幌
  • Year and Date: 2015-11-28
  • Invited
• [Presentation] 高純度アルギン酸ゲルと多段階分化誘導法を併用したマウスiPS細胞軟骨分化誘導法の確立 (2015)
  • Author(s): 本谷和俊、小野寺智洋、寺島理代、門間太輔、松岡正剛、馬場力哉、上徳善太、松原新史、宝満健太郎、岩崎倫政
  • Organizer: 第30回 日本整形外科学会基礎学術集会
  • Place of Presentation: 富山
  • Year and Date: 2015-10-22
• [Presentation] An acellular cartilage tissue repair technique using an ultrapurified alginate gel for the treatment of rabbit osteochondral defects (2015)
  • Author(s): Iwasaki N, Baba R, Momma D, Matsuoka M, Hontani K, Onodera T
  • Organizer: Biomaterials International 2015
  • Place of Presentation: Kenting,Taiwan
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] Chondrogenic differentiation of mouse Induced pluripotent stem cells (iPSCs) using ultra-purified alginate gel (UPAL gel) (2015)
  • Author(s): Hontani K, Onodera T, Momma D, Matsuoka M, Baba R, Iwasaki N
  • Organizer: International Cartilage Repair Society 2015
  • Place of Presentation: Chicago,IL,USA
  • Year and Date: 2015-05-08
  • Int'l Joint Research
• [Presentation] ultra-purified alginate gel (UPAL gel) を用いたマウス人工多能性幹細胞(iPS細胞)からの軟骨細胞分化誘導 (2015)
  • Author(s): 本谷和俊、小野寺智洋、岩崎倫政、他
  • Organizer: 第28回日本軟骨代謝学会
  • Place of Presentation: 東京医科歯科大学(東京都)
  • Year and Date: 2015-03-06 – 2015-03-07