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Establishment of a novel method for heat-stimulus induced osteogenesis using magnetite nanoparticles

Research Project

Project/Area Number 26670659
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionNagoya University

Principal Investigator

Yoshihiro Nishida  名古屋大学, 医学系研究科, 准教授 (50332698)

Co-Investigator(Kenkyū-buntansha) 小澤 英史  名古屋大学, 医学部附属病院, 病院助教 (60635572)
新井 英介  名古屋大学, 医学部附属病院, 病院助教 (40612841)
生田 国大  名古屋大学, 医学部附属病院, 医員 (40732657)
加藤 竜司  名古屋大学, 創薬科学研究科, 准教授 (50377884)
本多 裕之  名古屋大学, 予防早期医療創成センター, 教授 (70209328)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords温熱療法 / 骨形成 / 動物モデル / 人工骨 / 骨欠損 / 再生医学 / 医療・福祉 / 臨床
Outline of Final Research Achievements

New bone formation was significantly increased by hyperthermia using magnetite cationic liposome embedded in alginate beads with an alternating magnetic field (AMF) in a rat tibial defect model. Heating materials were changed to Resovist containing ferucarbotoran and hydroxyapatite scaffold, which have been already approved in clinical use in Japan. Combination materials were grafted in tibial defect of rat and rabbit and subjected to the multiple hyperthermia in AMF. Results demonstrated that newly formed bone was observed in the grafted materials from the periphery toward the center, and more osteoclasts were found in the once a week hyperthermia group compared with non-hyperthermia group.
Experiments of hyperthermia were performed in vitro using ATDC-5 and MC3T3 cells. Heat stress also induced enhanced alkaline phosphatase expression in cultured osteoblastic cells, MC3T3. On the other hand, heat stress had no obvious effects on chondrogenic differentiation using ATDC5 cells.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] In vivo heat-stimulus-triggered osteogenesis.2015

    • Author(s)
      Ikuta K, Urakawa H, Kozawa E, Hamada S, Ota T, Kato R, Honda H, Kobayashi T, Ishiguro N, Nishida Y.
    • Journal Title

      Int J Hyperthermia.

      Volume: 31(1): Issue: 1 Pages: 58-66

    • DOI

      10.3109/02656736.2014.988662

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Multiple heat stimuli enhanced osteogenesis of two species in vivo models2017

    • Author(s)
      Takehiro Ota, Hiroshi Urakawa, Eiji Kozawa, Kunihiro Ikuta, Shunsuke Hamada, Tomohisa Sakai, Naoki Ishiguro, Yoshihiro Nishida
    • Organizer
      2017 Orthopaedic Research Society Annual Meeting
    • Place of Presentation
      San Diego(USA)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 温熱刺激による新規骨形成法 ー臨床応用に向けてー2016

    • Author(s)
      生田国大 石黒直樹 西田佳弘
    • Organizer
      第30回東海ハイパーサーミア研究会
    • Place of Presentation
      名古屋市立大学医学部同窓会館(愛知県名古屋市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 温熱刺激による新規骨形成促進法2015

    • Author(s)
      生田国大, 浦川浩, 小澤英史, 濱田俊介, 石黒直樹, 西田佳弘
    • Organizer
      第124回中部日本整形外科災害外科学会・学術集会
    • Place of Presentation
      ホテル日航金沢(石川県金沢市)
    • Year and Date
      2015-04-10
    • Related Report
      2015 Research-status Report
  • [Presentation] In Vivo Heat-stimulus Triggered Osteogenesis2015

    • Author(s)
      Ikuta K, Urakawa H, Kozawa E, Hamada S, Ishiguro N, Nishida Y.
    • Organizer
      2015 Annual Meeting of Orthopedic Research Society,
    • Place of Presentation
      MGM Grand Hotel (米国ラスベガス)
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Research-status Report
  • [Presentation] 熱刺激による新規骨新生システム2014

    • Author(s)
      西田佳弘
    • Organizer
      中部地区 医療・バイオ系シーズ発表会
    • Place of Presentation
      ウインクあいち(名古屋市中村区)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 温熱刺激による新規骨形成促進法2014

    • Author(s)
      生田国大, 浦川浩, 新井英介, 小澤英史, 二村尚久, 濵田俊介, 大田剛広, 石黒直樹, 西田佳弘
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] Composition for new bone formation, and new bone formation system2014

    • Inventor(s)
      西田佳弘,生田国大,本多裕之,加藤竜司,小林猛
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-06-05
    • Acquisition Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 発熱による骨を申請させるシステム2014

    • Inventor(s)
      西田佳弘
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-06-05
    • Related Report
      2014 Research-status Report
    • Overseas

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Published: 2014-04-04   Modified: 2018-03-22  

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