| Project/Area Number |
26670662
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
FUJITA KAORI 京都大学, iPS細胞研究所, 特定研究員 (10633092)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUMAKI Noriyuki 京都大学, iPS細胞研究所, 教授 (50303938)
OHTA Akira 京都大学, iPS細胞研究所, 教授 (00168931)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 低分子化合物 / 軟骨細胞 / 脱分化 / 再分化 / 低分子化合物ライブラリー / siRNA ライブラリー |
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| Outline of Final Research Achievements |
We performed a small molecular weight compounds screen to identify compounds which re-differentiate dedifferentiated chondrocytes in monolayer-culture. A compound library consists of over 18,200 compounds introduced into the chondrogenic cell line (290-2-14)), which were directly converted from dermal fibroblasts of Col11a2-Egfp-Ires-Puro transgenic mice by inducible expression of c-Myc, Klf4 and Sox9 with doxycycline (Dox). We identified one candidate compound and analyzed its ability to re-differentiate dedifferentiated chondrocytes. Treatment of dedifferentiated chondrocytes with the candidate compound dose-dependently up-regulates chondrogenic marker genes (Col2a1 and Col11a2). Now, we have analyzed the detail molecular mechanisms for re-differentiation ability of the candidate compound.
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