| Project/Area Number |
26670665
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Hiroshi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (80325092)
OHTSUKI Takashi 岡山大学, 大学院保健学研究科, 非常勤研究員 (10534802)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 軟骨 / 培養細胞 |
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| Outline of Final Research Achievements |
The regulation of ADAMTS5 is crucial for osteoarthritis. However, little is known for its mechanism. We hypothesized that endocytosis may be involved in ADAMTS5 regulation. First, we found that endocytosis occurred in OUMS-27. We next examined endocytosis-related molecule, LRP-1 and RAP. The mRNA level of receptor-associated protein (RAP), antagonist of LRP-1, was not changed by IL-1 beta stimulation. It is interesting that the small-sized bands were found by Western blotting using anti-LRP-1 antibody after IL-1 beta stimulation. This is considered to be a short LRP-1 and the shedding of LRP-1 may be occurred by IL-1 beta stimulation.
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