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The strategy for refractory fracture using the combination of microRNA with magnetic targeting system

Research Project

Project/Area Number 26670666
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionHiroshima University

Principal Investigator

OCHI MITSUO  広島大学, その他部局等, 学長 (70177244)

Co-Investigator(Kenkyū-buntansha) MIYAKI SHIGERU  広島大学, 病院, 講師 (10392490)
KAMEI NAOSUKE  広島大学, 病院, 講師 (70444685)
NAKASA TOMOYUKI  広島大学, 病院, 病院助教 (60467769)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
KeywordsマイクロRNA / 磁気ターゲッティング / 難治性骨折
Outline of Final Research Achievements

It is difficult to achieve bone union for refractory fracture cases with a non- invasive technique. MicroRNAs (miRNAs) are short, non-coding RNAs that act as repressors of gene expression at the level of post transcriptional regulation. The aim of this study was to identify miRNA which play an important role in osteogenesis, and acceleration of bone union by regulating identified miRNA in rat refractory fracture model. miR-222 was identified by microarray analysis during osteogenesis. Inhibition of miR-222 promoted osteogenesis and over-expression of miR-222 inhibited osteogenesis from human mesenchymal stem cells in vitro. miR-222 inhibitor was administered into the fracture site in rat refractory fracture model. Bone union at the fracture site was achieved in the miR-222 inhibitor administration by confirming radiographic and histological evaluation. Local administration of miR-222 inhibitor could accelerate bone healing through enhancing osteogenesis in the rat refractory model.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] microRNA-222の発現抑制による骨形成促進の検討2015

    • Author(s)
      吉塚将昭、中佐智幸、川西啓生、蜂須賀晋、古田太輔、越智光夫
    • Organizer
      第58回日本手外科学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2015-04-16
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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