| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
By forcing c-Myc expression and performing ChIP-sequence in that state, we have analyzed and verified data by microRNA and cDNA array. Currently it is likely that let-7a, miR-16, miR-29b, miR-1294, and/or miR-2682 are involved. We demonstrated that the transfection of these two factors suppresses the expression of mRNA and protein on c-Myc, and also suppresses the progression of the cell cycle.To examine the presence or absence of chromosomal instability during the study period, we are attempting to detect abnormalities in genome fraction and in the number of DNA copies using the DNA ploidy analysis and array-CGH. Our prediction is that one of the DSB repair systems, homologous recombination or non-homologous end joining, has a greater dysfunction. We are seeking to determine this through HR activity measurement in c-myc-introduced fibroblasts by I-Scel, and random plasmid integration, which is an NHEJ assay.
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