Elucidating mechanisms of altered bone matrix mineralization

• Project/Area Number: 26670674
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: MATSUO Koichi 慶應義塾大学, 医学部, 教授 (40229422)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨基質 / ミネラリゼーション / 石灰化 / 骨芽細胞 / 基質小胞 / 骨代謝

Outline of Final Research Achievements

Bone matrix mineralization (calcification) proceeds through organized deposition of calcium hydroxyapatite crystals on collagen fibers. Both hyper- and hypomineralzation reduce bone mechanical properties. Mice lacking tyrosine kinase receptor EphA showed hypermineralization likely due to altered growth of mineralizing nodules.

Research Products

• [Int'l Joint Research] メルボルン大学/-/-(オーストラリア)
• [Int'l Joint Research] モントリオール大学/-/-(カナダ)
• [Int'l Joint Research] 香港大学/-/-(中国)
• [Journal Article] Osteocyte-directed bone demineralization along canaliculi. (2016)
  • Author(s): Nango N., Kubota S., Hasegawa T., Yashiro W., Momose A., Matsuo K.
  • Journal Title: Bone. Volume: 84 Pages: 279-288
  • DOI: 10.1016/j.bone.2015.12.006
  • Peer Reviewed / Open Access
• [Journal Article] EphB4 expressing stromal cells exhibit an enhanced capacity for hematopoietic stem cell maintenance. (2015)
  • Author(s): Thao Nguyen, Agnieszka Arthur, Romana Panagopoulos, Sharon Paton, John D. Hayball, Andrew Zannettino, Louise Purton, Koichi Matsuo, and Stan Gronthos.
  • Journal Title: Stem Cells. Volume: 33 Issue: 9 Pages: 2838-2849
  • DOI: 10.1002/stem.2069
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice. (2015)
  • Author(s): Valverde-Franco G, Hum D, Matsuo K, Lussier B, Pelletier JP, Fahmi H, Kapoor M, Martel-Pelletier J.
  • Journal Title: Am J Pathol. Volume: 185 Issue: 2 Pages: 335-346
  • DOI: 10.1016/j.ajpath.2014.10.004
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Continuous Parathyroid Hormone Injection in Mouse Has Differential Effects on Osteoclast Activation in Primary and Secondary Spongiosa. (2015)
  • Author(s): Nango Nobuhito, Kubota Shogo, Yashiro Wataru, Momose Atsushi, Ichinose Shizuko, Matsuo Koichi.
  • Organizer: Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: シアトル（米国）
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] EphB/ephrin-B interactions regulate stromal cell fate determination and bone marrow support. (2015)
  • Author(s): Gronthos Stan, Nguyen Thao, Purton Louise, Matsuo Koichi, Arthur Agnes.
  • Organizer: Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: シアトル（米国）
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Expression of Eph-ephrin signalling in osteocytes under mechanical loading. (2015)
  • Author(s): Chongshan LIAO, Chengfei ZHANG, Lijian JIN, Koichi MATSUO, Yanqi YANG.
  • Organizer: 91st Congress of the European Orthodontic Society
  • Place of Presentation: ベニス（イタリア）
  • Year and Date: 2015-06-13
  • Int'l Joint Research
• [Presentation] Influence of lipopolysaccharide on ephrin/Eph expression in human periodontal ligament fibroblasts. (2015)
  • Author(s): Minjie Li, Chengfei Zhang, Lijian Jin, Koichi Matsuo, Yanqi Yang.
  • Organizer: 91st Congress of the European Orthodontic Society
  • Place of Presentation: ベニス（イタリア）
  • Year and Date: 2015-06-13
  • Int'l Joint Research
• [Remarks] 慶應義塾大学医学部 松尾光一（細胞組織学）研究室
  • URL: http://wp4.matsuo-lab.com