Reoxygenation with 100% oxygen following hypoxia in newborn mice causes learning impairment
| Project/Area Number |
26670690
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Kobe University |
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Principal Investigator |
UEKI MASAAKI 神戸大学, 医学(系)研究科(研究院), 研究員 (20213332)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 新生児蘇生 / 100％酸素 / 迷路学習 / エリスロポイエチン / 低酸素 / 100％酸素蘇生 / 脳発達障害 / caspase-3 / 8方向迷路 |
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| Outline of Final Research Achievements |
Postnatal day 7 (P7) mice were either subjected to hypoxia in 8% oxygen for 60 min or served as controls. Following hypoxia, mice underwent reoxygenation for 30 min with 21% or 100% oxygen. Reoxygenation with 100% oxygen significantly increased caspase-3 in the whole brain compared with 21% oxygen, and significantly decreased erythropoietin (EPO) mRNA expression compared with 21% oxygen 9 h after reoxygenation. There was no difference in brain-derived neurotrophic factor mRNA levels among the groups. The performance ratios of error in mice given 100% oxygen in the radial maze test at P63 were significantly higher compared with mice given 21% oxygen. Furthermore, administration of EPO (5000 u/kg), EUK134 (20mg/kg) rescued the performance ratios of error in mice given 100% oxygen in the radial maze test.
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Report
(2 results)
Research Products
(2 results)
