| Project/Area Number |
26670702
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Okayama University |
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Principal Investigator |
Kumon Hiromi 岡山大学, 医歯(薬)学総合研究科, 特命教授 (30144760)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA Eiji 岡山大学, 大学院医歯薬学総合研究科, 教授 (20181688)
ARAKI Motoo 岡山大学, 病院, 講師 (90467746)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 石灰化小球 / 酸化脂質 / 質量分析 / イメージング質量分析 / 異所性石灰化 / バイオテクノロジー / 脂質 / 臨床 / ストレス / 微生物 |
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| Outline of Final Research Achievements |
Our study has revealed the components, especially, oxidized lipids derived from γ-irradiated FBS can act as a scaffold to generate so-called “Nanobacteria-like particles (NLP)”. Lipids were extracted from three different experimental groups and were subjected to be separated on TLC plate. Individual spot was collected and further analyzed with MALDI-TOF-MS, while the oxidized lipids were characterized with MS/MS. As a result, lyso-PC was detected in NLP grown in γ-irradiated FBS. Nevertheless, 7-ketocholesterol and oxLig-1 (7-ketocholesteryl 9-carboxynonanoate) were found abundantly in both γ-irradiated FBS itself and NLP grown with non-γ-irradiated FBS in the presence of oxLig-1-liposomes. Subsequent characterization of those NLP specimens by scanning electron microscopy revealed that NLPs grown in γ-irradiated FBS demonstrated rounded shapes with larger dimensions than those in non-γ-irradiated FBS.
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