| Project/Area Number |
26670712
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
Abe Hiroyuki 山形大学, 大学院理工学研究科, 教授 (10375199)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
渡部 裕輝 山形大学, 大学院理工学研究科, 准教授 (00333328)
|
|---|
| Research Collaborator |
KUROTANI Reiko
TAKAKURA Kei
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 光干渉断層撮影 / 卵母細胞 / 発生 / 加齢 / 生殖医療 |
|---|
| Outline of Final Research Achievements |
In this study, we have examined optical coherence tomography (OCT) for noninvasive analysis of follicular development in mice ovaries. Ovary has many follicles and oocytes at different developmental stages. Quantification of the follicle at various developmental stages is important to evaluate the capacity of the ovary to provide oocytes that are capable of fertilization resulting in a successful pregnancy. 3D structural OCT images were identified each developmental stage from primary follicle (50 μm in diameter) to antral follicle (350 μm in diameter) in the ovary of 25.5-day-old mouse. Further we found time-varying OCT signals at the oocytes compared with around tissues. The motions of oocytes were clearly enhanced by inter-frame intensity based Doppler OCT techniques. The OCT techniques are effective to analyze the development of follicles and oocytes in ovaries.
|
