| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
To identify the stem cell of endometriosis, we first isolated epithelial and stromal cells from ovarian endometriomas resected by operation. The hTERT, cyclinD1 and CDK4 cDNAs were transfected to these cells by lentiviral transfection, and immortalized cells were successfully isolated. Using these cells, FACS analysis was performed, in which the cells with various cell surface markers were analyzed for invasion and sphere formation capacities. We found that CD44-positive cells have increased invasive ability and obtain efficient sphere formation activity, and therefore contain stem-cell population.
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