Identification of endometriosis-stem cells for the development of novel molecular-target therapy
Project/Area Number |
26670718
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
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Research Institution | Shimane University |
Principal Investigator |
SATORU KYO 島根大学, 医学部, 教授 (50272969)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 子宮内膜症 / 幹細胞 / 癌化 |
Outline of Final Research Achievements |
To identify the stem cell of endometriosis, we first isolated epithelial and stromal cells from ovarian endometriomas resected by operation. The hTERT, cyclinD1 and CDK4 cDNAs were transfected to these cells by lentiviral transfection, and immortalized cells were successfully isolated. Using these cells, FACS analysis was performed, in which the cells with various cell surface markers were analyzed for invasion and sphere formation capacities. We found that CD44-positive cells have increased invasive ability and obtain efficient sphere formation activity, and therefore contain stem-cell population.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] KRAS/BRAF Analysis in Ovarian Low-Grade Serous Carcinoma Having Synchronous All Pathological Precursor Regions.2016
Author(s)
Nakamura K, Nakayama K, Ishibashi T, Ishikawa N, Ishikawa M, Katagiri H, Minamoto T, Sato E, Sanuki K, Yamashita H, Iida K, Sultana R, Kyo S.
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Journal Title
Int J Mol Sci.
Volume: 17
Issue: 5
Pages: 625-625
DOI
Related Report
Peer Reviewed / Open Access
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