| Project/Area Number |
26670728
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyorin University (2015)
Keio University (2014) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Maruyama Tetsuo 慶應義塾大学, 医学部産科婦人科学教室, 准教授 (10209702)
Ota Kuniaki 慶應義塾大学, 医学部産科婦人科学教室, 研究員 (90424142)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 常位胎盤早期剥離 / リガンド / BMPR2 / BMP4 / BMP7 / 核磁気共鳴法 |
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| Outline of Final Research Achievements |
To clarify pathological mechanisms and clinical targets of placental abruption, we researched signal transduction concerned BMPR2 in pregnant uteri. First, we succeed to transfect mouse Bmpr2 with tag gene in NIH3T3 cells and extract high concentrated BMPR2/tag fusion protein from the NIH3T3 cells by immunoprecipitation. Second, we narrowed ligands candidate of mouse BMPR2 by mixture of the purified fusion protein solution and extracted protein solution from pregnant mouse uteri. As a result, we detected BMP4 and BMP7 as ligands candidate of mouse BMPR2 in pregnant uteri.
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