| Project/Area Number |
26670730
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | St. Marianna University School of Medicine |
|---|
Principal Investigator |
OHTA Tomohiko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (60233136)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | BRCA1 / エストロゲン / 卵管癌 / DNA損傷 / ヘテロクロマチン |
|---|
| Outline of Final Research Achievements |
The mechanism of tissue specificity; how BRCA1 mutation specifically triggers only breast and ovarian cancer, has been unrevealed. In this study we established doxycyclin-inducible BRCA1 deficient and estrogen receptor α (ERα) positive diploid human breast cell lines and investigated the effect of estrogen receptor expression on genomic and epigenomic stability in BRCA1-defective cellular background. BRCA1-depleted/ERα-positive cells exhibited chromatin de-compaction, increase of G2/M phase population and apoptosis, accompanied by significant cell number reduction, in response to doxycyclin treatment when compared to control BRCA1-depleted or ERα-positive cells.
|
