Unravelling the mechanism of ovarian carcinogenesis caused by BRCA1 depletion and estrogen action
| Project/Area Number |
26670730
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
OHTA Tomohiko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (60233136)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | BRCA1 / エストロゲン / 卵管癌 / DNA損傷 / ヘテロクロマチン |
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| Outline of Final Research Achievements |
The mechanism of tissue specificity; how BRCA1 mutation specifically triggers only breast and ovarian cancer, has been unrevealed. In this study we established doxycyclin-inducible BRCA1 deficient and estrogen receptor α (ERα) positive diploid human breast cell lines and investigated the effect of estrogen receptor expression on genomic and epigenomic stability in BRCA1-defective cellular background. BRCA1-depleted/ERα-positive cells exhibited chromatin de-compaction, increase of G2/M phase population and apoptosis, accompanied by significant cell number reduction, in response to doxycyclin treatment when compared to control BRCA1-depleted or ERα-positive cells.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] NF-kB signaling mediates acquired resistance after PARP inhibition.2015
Author(s)
Nakagawa Y, Sedukhina AS, Okamoto N, Nagasawa S, Suzuki N, Ohta T, Hattori H, Roche-Molina M, Narváez AJ, Jeyasekharan AD, Bernal JA, Sato K.
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Journal Title
Oncotarget.
Volume: 6
Pages: 3825-3839
Related Report
Peer Reviewed
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[Journal Article] LSD1 Overexpression Is Associated with Poor Prognosis in Basal-Like Breast Cancer, and Sensitivity to PARP Inhibition.2015
Author(s)
Nagasawa S, Sedukhina AS, Nakagawa Y, Maeda I, Kubota M, Ohnuma S, Tsugawa K, Ohta T, Roche-Molina M, Bernal JA, Narváez AJ, Jeyasekharan AD, Sato K.
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Journal Title
PLoS One.
Volume: 10
Issue: 2
Pages: e0118002-e0118002
DOI
Related Report
Peer Reviewed
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