Mechanism of sperm survival regulated by male and female liquid factors.
Project/Area Number |
26670732
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
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Research Institution | Meiji University (2015-2016) National Research Institute for Child Health and Development (2014) |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
MIYADO Kenji (国)国立成育医療研究センター, 細胞医療研究部, 室長 (60324844)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 生殖免疫 / 子宮内殺精子因子 / 補体 / 精漿タンパク質 / 子宮内細菌 / 体内受精の免疫 / 性周期 |
Outline of Final Research Achievements |
At the beginning of this research, steroid hormones were expected to define the sperm survival in female uterus; however, any sperm-killing inducible steroid hormone was not detected in the uterine fluid. The shotgun analysis of the uterine fluid by using LC/MS revealed that complement C3 existed with extremely high concentration if the fluid showed spermicide. C3 was highly expressed in the uterus if the mouse was in estrus period; but C3 was neither in diestrus period nor after injection of hCG. From ovariectomized mice, the secretion of C3 in the uterus was under controlled by estradiol. Both immuno-depletion of C3 and heat inactivation (56°C for 30 min) of the uterine secretion decreased the sperm killing effect. A mass of complement C3 in the uterus was easy to detect by western blotting; but the incubation with sperm could not activate uterine C3. The sperm killing mechanism in uterus continues to be investigated.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Birth weights and Down syndrome in neonates delivered after frozen-thawed embryo transfer: Six-year study (2007-2012) on the national assisted reproduction registry data in Japan2017
Author(s)
Yamatoya K, Saito K, Saito T, Kang W, Nakamura A, Miyado M, Kawano N, Miyamoto Y, Umezawa A, Miyado K, Saito H
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Journal Title
Reproductive Medicine and Biology
Volume: 16
Issue: 2
Pages: 228-234
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mitochondrial Fission Factor Drp1 Maintains Oocyte Quality via Dynamic Rearrangement of Multiple Organelles2014
Author(s)
O Udagawa, T Ishihara, M Maeda, Y Matsunaga, S Tsukamoto, N Kawano, K Miyado, H Shitara, S Yokota, M Nomura, K Mihara, N Mizushima, N Ishihara
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Journal Title
Current Biology
Volume: 24
Issue: 20
Pages: 2451-2458
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Seminal vesicle protein SVS2 is required for sperm survival in the uterus2014
Author(s)
N Kawano, N Araki, K Ybshida, THibino, N Ohnami, M Makino, S Kanai, H Hasuwa, M Yoshida, K Miyado, A Umezawa
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 111(11)
Issue: 11
Pages: 4145-4150
DOI
Related Report
Peer Reviewed / Open Access
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