autoantibody-targeted proteomics for recurrent pregnancy loss

Research Project

Project/Area Number	26670735
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Obstetrics and gynecology
Research Institution	Osama Woman's and Children's Hospital
Principal Investigator	WADA YOSHINAO 地方独立行政法人大阪府立病院機構大阪母子医療センター(研究所), その他部局等, 母性内科・医師 (00250340)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000) Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	不育症 / 自己抗体 / トランスグルタミナーゼ / 自己免疫疾患 / 胎児発育不全 / 胎児発育遅延 / セリアック病
Outline of Final Research Achievements	We investigated the possibility that anti-transglutaminase 2 (TG2) autoantibodies can be a cause of recurrent pregnancy loss. ELISA of anti-TG2-IgG antibodies searched on 83 sera of systemic lupus erythematosus and Sjogren's syndrome found two patients to be positive. Binding to TG2 was confirmed by Western blotting using recombinant TG2 as an antigen. One of the patients had recurrent miscarriage which was unresponsive to anti-coagulation. Both patients had no celiac disease-like digestive symptoms. Twelve patients with recurrent pregnancy loss of unknown causes were anti-TG2 antibody negative. From these results, it was found that the anti-TG2 antibodies may be present in parallel with other autoantibodies. However, it was not possible to draw conclusions about the anti-TG2 antibody as a cause of recurrent pregnancy loss.